Tailoring of peroxidase mimetics bifunctional nanocomposite: Dual mode electro-spectroscopic screening of cholesterol and hydrogen peroxide in real food samples and live cells

Peroxidase mimetics of bifunctional nanocomposite for dual mode electro-spectroscopic screening of cholesterol and hydrogen peroxide in real food samples and live cells. [Display omitted] •Developed a dual biosensing platform for cholesterol and H2O2 using ChOx-BSA-AuNPs-MFOF.•The platform displayed a wide linear range concentration and nanomolar level detection limit.•The platform was found to be easy to use, rapid, interference-free, highly stable, and reproducible.•The platform was capable of detecting Chol and H2O2 in real food samples and live cells.•To comply with JECFA and FAD guidelines for analyzing Chol in real food and juice samples. A simple and rapid screening of biomarkers in clinical and food matrices is urgently needed to diagnose cardiovascular diseases. The cholesterol (Chol) and hydrogen peroxide (H2O2) are critical bio-indicators, which require more inventive detection techniques to be applied to real food, and bio-samples. In this study, a robust dual sensor was developed for Chol and H2O2 using hybrid catalyst. Bovine serum albumin (BSA)-capped nanocatalyst was potentially catalyzed 3,3′,5,5′-tetramethylbenzidine (TMB), and H2O2. The enzymatic nanoelectrocatalyst delivered a wide range of signaling concentrations from 250 nM to 3.0 mM and 100 nM to 10 mM, limit of detection (LOD) of 53.2 nM and 18.4 nM for Chol and H2O2. The cholesterol oxidase-BSA-AuNPs-metal-free organic framework (ChOx-BSA-AuNPs-MFOF) based electrode surface effectively operated in live-cells and real-food samples. The enzymatic sensor exhibits adequate recovery of real-food samples (96.96–99.44%). Finally, the proposed system is a suitable choice for the potential applications of Chol and H2O2 in clinical and food chemistry.