Thermomonas paludicola sp. nov., isolated from a lotus wetland

A Gram-stain-negative, aerobic, rod-shaped and motile bacterium, designated IMCC34681 , was isolated from a lotus wetland in the Republic of Korea. Cellular growth occurred at 10-37 °C (optimum, 30 °C), pH 6-9 (optimum, pH 7) and with 0-2 % (w/v) NaCl (optimum, 0.5 % NaCl). The results of 16S rRNA gene sequence analysis indicated that IMCC34681 represented a member of the genus , sharing 95.3-96.9 % similarities with type strains of species of the genus. The whole-genome sequence of IMCC34681 was 2.72 Mbp in size with 66.2 % DNA G+C content. The IMCC34681 genome shared the highest average nucleotide identity (ANI) value, 82.8 %, with that of KACC 16975 among species of the genus , indicating that the strain represents a novel genomic species. The major respiratory quinone of the strain was ubiquinone-8 (Q-8) and the predominant cellular fatty acids were iso-C (25.7 %) and iso-C (20.8 %). The strain harboured diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unidentified lipid as major fatty polar lipids. On the basis of the phylogenetic, phenotypic, chemotaxonomic and genomic characteristics, IMCC34681 was assigned to the genus as the type strain of a novel species, for which the name sp. nov. is proposed. The type strain is IMCC34681 (=KACC 21793 =NBRC 114635 ).