Fructilactobacillus cliffordii sp. nov. , Fructilactobacillus hinvesii sp. nov., Fructilactobacillus myrtifloralis sp. nov., Fructilactobacillus carniphilus sp. nov. and Fructobacillus americanaquae sp. nov., five novel lactic acid bacteria isolated from insects or flowers of Kangaroo Island, South Australia

Six strains, KI11_D11 , KI4_B1, KI11_C11 , KI16_H9 , KI4_A6 and KI3_B9 , were isolated from insects and flowers on Kangaroo Island, South Australia. On the basis of 16S rRNA gene phylogeny, strains KI11_D11 , KI4_B1, KI11_C11 , KI16_H9 , KI4_A6 were found to be closely related to Ru20-1 . Due to the lack of a whole genome sequence for this species, whole genome sequencing of Ru20-1 was undertaken. KI3_B9 was found to be closely related to F214-1 . Utilizing core gene phylogenetics and whole genome analyses, such as determination of AAI, ANI and dDDH, we propose that these six isolates represent five novel species with the names (KI11_D11 = LMG 32130 = NBRC 114988 ), (KI11_C11 = LMG 32129 = NBRC 114987 ), (KI16_H9 = LMG 32131 = NBRC 114989 ) (KI4_A6 = LMG 32127 = NBRC 114985 ) and (KI3_B9 = LMG 32124 = NBRC 114983 ). Chemotaxonomic analyses detected no fructophilic characters for these strains of member of the genus . KI3_B9 was found to be obligately fructophilic, similarly to its phylogenetic neighbours in the genus . This study represents the first isolation, to our knowledge, of novel species in the family from the Australian wild.