STAT6 controls the stability and suppressive function of regulatory T cells

Signal transducer and activator of transcription 6 (STAT6) promotes tumorigenesis by decreasing the Forkhead box P3+ (Foxp3+) cell frequency allowing for the infiltration of inflammatory cells during the early stages of colitis‐associated cancer (CAC). In this study, we dissected the role of STAT6 in the generation of inducible in vitro regulatory T cells (iTregs) and peripheral in vivo Tregs (pTregs) under inflammatory conditions. In in vitro assays, when STAT6 was lacking, iTregs preserved a stable phenotype and expressed high levels of Foxp3 and CD25 during long expansion periods, even in the presence of IL‐6. This effect was associated with increased in vitro suppressive ability, over‐expression of programmed death‐1 (PD‐1), CTLA‐4, and Foxp3, and decreased IFN‐γ expression. Furthermore, iTregs developed during STAT6 deficiency showed a higher demethylation status for the FOXP3 Treg‐specific demethylated region (TSDR), coupled with lower DNA methyltransferase 1 (DNMT1) mRNA expression, suggesting that STAT6 may lead to Foxp3 silencing. Using a mouse model of CAC, the STAT6‐/‐ pTregs expressed a more activated phenotype at the intestine, had higher suppressive capacity, and expressed more significant levels of PD‐1 and latency‐associated peptide of TGF‐β (LAP) associated with their ability to attenuate tumor development. These data suggest that STAT6 signaling impairs the induction, stability, and suppressive capacity of Tregs developed in vitro or in vivo during gut inflammation. Through a series of in vitro and in vivo experiments, we have demonstrated that STAT6 signaling is crucial for controlling Tregs stability and suppressive function by modulating the Treg‐specific demethylated region (TSDR) methylation state of the FOXP3 gene in homeostatic and inflammatory conditions.