Unravelling ciprofloxacin removal in a nitrifying moving bed biofilm reactor: Biodegradation mechanisms and pathways

Although moving bed biofilm reactors (MBBRs) have shown excellent antibiotic removal potentials, the information on underlying mechanisms is yet limited. This work assessed the removal of ciprofloxacin in an enriched nitrifying MBBR by clarifying the contribution of adsorption and microbial-induced biodegradation. Results demonstrated the considerable biomass adsorption (55%) in first 30 min. Limiting nitrite oxidizing bacteria growth or inhibiting nitrification would lead to lower adsorption capacities. The highest ciprofloxacin biodegradation rate constant was 0.082 L g SS−1 h−1 in the presence of ammonium, owing to ammonia oxidizing bacteria (AOB)-induced cometabolism, while heterotrophs played an insignificant role (∼9%) in ciprofloxacin biodegradation. The developed model also suggested the importance of AOB-induced cometabolism and metabolism over heterotrophs-induced biodegradation by analyzing the respective biodegradation coefficients. Cometabolic biodegradation pathways of ciprofloxacin mainly involved the piperazine ring cleavage, probably alleviating antimicrobial activities. It implies the feasibility of nitrifying biofilm systems towards efficient antibiotic removal from wastewater. [Display omitted] •Biofilm adsorption had a considerable contribution to ciprofloxacin removal.•AOB-induced cometabolism played an important role in ciprofloxacin biodegradation.•Heterotrophs had minor contributions to ciprofloxacin biodegradation.•The key parameters related to ciprofloxacin biodegradation model were estimated.•Cometabolic biodegradation pathways mainly involved the piperazine ring cleavage.