Correlative increasing expressions of KIF5b and Nav1.7 in DRG neurons of rats under neuropathic pain conditions

•KIF5b, one isoform of kinesin-1, was necessary for the membrane localizations of Nav1.7 in DRG neurons.•KIF5b interacted with Nav1.7 and mediated the trafficking of Nav1.7.•Intrathecal injections of KIF5b shRNA moderated the SNI-induced both mechanical and thermal hyperalgesia.•The rescued analgesi...

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Veröffentlicht in:Physiology & behavior 2023-05, Vol.263, p.114115-114115, Article 114115
Hauptverfasser: Yin, Jun-Bin, Liu, Hai-Xia, Dong, Qin-Qin, Wu, Huang-Hui, Liang, Zhuo-Wen, Fu, Jin-Tao, Zhao, Wen-Jun, Hu, Huai-Qiang, Guo, Hong-Wei, Zhang, Ting, Lu, Ya-Cheng, Jin, Shan, Wang, Xiao-Ling, Cao, Bing-Zhen, Wang, Zhe, Ding, Tan
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Sprache:eng
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Zusammenfassung:•KIF5b, one isoform of kinesin-1, was necessary for the membrane localizations of Nav1.7 in DRG neurons.•KIF5b interacted with Nav1.7 and mediated the trafficking of Nav1.7.•Intrathecal injections of KIF5b shRNA moderated the SNI-induced both mechanical and thermal hyperalgesia.•The rescued analgesic effects of KIF5b shRNA also alleviated SNI-induced negative emotional behaviors. Nav1.7, one of tetrodotoxin-sensitive voltage-gated sodium channels, mainly expressed in the small diameter dorsal root ganglion (DRG) neurons. The expression and accumulation on neuronal membrane of Nav1.7 increased following peripheral tissue inflammation or nerve injury. However, the mechanisms for membrane accumulation of Nav1.7 remained unclear. We report that KIF5b, a highly expressed member of the kinesin-1 family in DRGs, promoted the translocation of Nav1.7 to the plasma membrane in DRG neurons of the rat. Following nociceptive behaviors in rats induced by peripheral spared nerve injury (SNI), synchronously increased KIF5b and Nav1.7 expressions were observed in DRGs. Immunohistochemistry staining demonstrated the co-expressions of KIF5b and Nav1.7 in the same DRG neurons. Immunoprecipitation experiments further confirmed the interactions between KIF5b and Nav1.7. Moreover, intrathecal injections of KIF5b shRNA moderated the SNI-induced both mechanical and thermal hyperalgesia. The rescued analgesic effects also alleviated SNI-induced anxiety-like behaviors. In sum, KIF5b was required for the membrane localizations of Nav1.7, which suggests a novel mechanism for the trafficking of Nav1.7 involved in neuropathic pain.
ISSN:0031-9384
1873-507X
DOI:10.1016/j.physbeh.2023.114115