Precorneal retention time of ocular lubricants measured with fluorophotometry in healthy dogs

Objective Determine the precorneal retention time of five different ocular lubricants commonly used in dogs. Animals Studied Six healthy Beagle dogs (n = 12 eyes). Procedures Five ocular lubricants were studied: Artificial Tears Solution® (1.4% polyvinyl alcohol), I‐Drop® Vet Plus (0.25% hyaluronate), Optixcare® Eye Lube Plus (0.25% hyaluronate), Systane® Ultra (0.4% polyethylene glycol 400 and 0.3% propylene glycol), and Artificial Tears Ointment® (mineral oil/white petrolatum). Each lubricant was mixed with 10% sodium fluorescein to achieve 1% fluorescein formulations. Following topical administration of 35 mg in each eye, tear fluid was collected with capillary tubes at selected times (0, 1, 5, 10, 20, 30, 40, 50, 60, 90, 120, 180 min) and fluorescein concentrations were measured with a computerized scanning ocular fluorophotometer. Results Tear fluorescence was significantly greater with Artificial Tears Ointment® compared with other lubricant formulations from 1 to 20 min post‐administration. Median (range) precorneal retention times were significantly different among the 5 lubricants, ranging from 40 minutes (20–90 min) for Artificial Tears Ointment®, 35 min (20–90 min) for Systane® Ultra, 30 min (10–60 min) for I‐Drop® Vet Plus, 25 min (10–60 min) for Optixcare® Eye Lube Plus, and 10 min (10–20 min) for Artificial Tears Solution®. Precorneal retention time was significantly lower for Artificial Tears Solution® compared with the other 4 formulations. Conclusions This study established normative data for the retention time of common lubricants on the ocular surface of dogs, which may be used to guide clinicians with their choice of lubricant and frequency of administration.