A comparison of study on intestinal barrier protection of polysaccharides from Hericium erinaceus before and after fermentation
The intestinal barrier protects the host from harmful substances. This paper investigated two polysaccharides extracted from the Hericium erinaceus before and after fermentation (HEP and FHEP). The effects of two polysaccharides on the intestinal barrier were investigated in cell and mice models. Th...
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| Veröffentlicht in: | International journal of biological macromolecules 2023-04, Vol.233, p.123558-123558, Article 123558 |
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| container_title | International journal of biological macromolecules |
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| creator | Su, Yue Cheng, Shasha Ding, Yixin Wang, Linge Sun, Mingshuang Man, Chaoxin Zhang, Yu Jiang, Yujun |
| description | The intestinal barrier protects the host from harmful substances. This paper investigated two polysaccharides extracted from the Hericium erinaceus before and after fermentation (HEP and FHEP). The effects of two polysaccharides on the intestinal barrier were investigated in cell and mice models. The results showed that polysaccharides had a protective effect against acrylamide-induced injury in IEC-6 cell. Compared with HEP, FHEP significantly increased TEER and paracellular permeability (P |
| doi_str_mv | 10.1016/j.ijbiomac.2023.123558 |
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[Display omitted]
•FHEP had a more significant protective effect in vitro and in vivo.•FHEP could better prevent the acrylamide induced damage of intestinal barrier.•HEP and FHEP could maintain the intestinal immune barrier of mice.•HEP and FHEP were found to increase the abundance of beneficial bacteria.•HEP and FHEP could improve the intestinal barrier function.</description><identifier>ISSN: 0141-8130</identifier><identifier>EISSN: 1879-0003</identifier><identifier>DOI: 10.1016/j.ijbiomac.2023.123558</identifier><identifier>PMID: 36746300</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Fermentation ; Fermented ; Gut microbiota ; Intestinal barrier ; Intestinal Mucosa - metabolism ; Intestines ; Mice ; Polysaccharide ; Polysaccharides - metabolism ; Polysaccharides - pharmacology</subject><ispartof>International journal of biological macromolecules, 2023-04, Vol.233, p.123558-123558, Article 123558</ispartof><rights>2023</rights><rights>Copyright © 2023. Published by Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c368t-d60a2bac55d6af0e39ff1afa89b1f2edf0fa5b6bf9b587b1da9a11aa679ece0c3</citedby><cites>FETCH-LOGICAL-c368t-d60a2bac55d6af0e39ff1afa89b1f2edf0fa5b6bf9b587b1da9a11aa679ece0c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ijbiomac.2023.123558$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36746300$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Su, Yue</creatorcontrib><creatorcontrib>Cheng, Shasha</creatorcontrib><creatorcontrib>Ding, Yixin</creatorcontrib><creatorcontrib>Wang, Linge</creatorcontrib><creatorcontrib>Sun, Mingshuang</creatorcontrib><creatorcontrib>Man, Chaoxin</creatorcontrib><creatorcontrib>Zhang, Yu</creatorcontrib><creatorcontrib>Jiang, Yujun</creatorcontrib><title>A comparison of study on intestinal barrier protection of polysaccharides from Hericium erinaceus before and after fermentation</title><title>International journal of biological macromolecules</title><addtitle>Int J Biol Macromol</addtitle><description>The intestinal barrier protects the host from harmful substances. This paper investigated two polysaccharides extracted from the Hericium erinaceus before and after fermentation (HEP and FHEP). The effects of two polysaccharides on the intestinal barrier were investigated in cell and mice models. The results showed that polysaccharides had a protective effect against acrylamide-induced injury in IEC-6 cell. Compared with HEP, FHEP significantly increased TEER and paracellular permeability (P < 0.05). Both polysaccharides the expression of alter tight junction (TJ) and mucin (MUC) as observed in cell Western Bolt (WB). Polysaccharides also enhance the intestinal barrier function in mice by improving cyclophosphamide induced cytokines level, TJ and MUC expression, and gut microbiota. The results showed that FHEP significantly increased IgA, IgG, and IgM levels while decreasing TNF-, IL-1, and IL-6 levels (P < 0.05). The immunohistochemical results showed that both polysaccharides significantly increased the expression of occludin, ZO-1, ZO-2, claudin-3, claudin-4, MUC2 and decreased claudin-2. In parallel, polysaccharides could alter the composition of the gut microbiota, indicating that increased in Bacteriodetes, Firmicutes and decreased in Klebsiella and Shigella. This work provides important views on the protective effect of fermented polysaccharides on the intestinal barrier, and provides a potential mechanism for the beneficial health properties of these biomacromolecules.
[Display omitted]
•FHEP had a more significant protective effect in vitro and in vivo.•FHEP could better prevent the acrylamide induced damage of intestinal barrier.•HEP and FHEP could maintain the intestinal immune barrier of mice.•HEP and FHEP were found to increase the abundance of beneficial bacteria.•HEP and FHEP could improve the intestinal barrier function.</description><subject>Animals</subject><subject>Fermentation</subject><subject>Fermented</subject><subject>Gut microbiota</subject><subject>Intestinal barrier</subject><subject>Intestinal Mucosa - metabolism</subject><subject>Intestines</subject><subject>Mice</subject><subject>Polysaccharide</subject><subject>Polysaccharides - metabolism</subject><subject>Polysaccharides - pharmacology</subject><issn>0141-8130</issn><issn>1879-0003</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM1LxDAQxYMoun78C5Kjl65Js03bmyJ-geBFz2GSTDBL26xJK-zJf90sXb16mmF47w3vR8glZ0vOuLxeL_1a-9CDWZasFEteiqpqDsiCN3VbMMbEIVkwvuJFwwU7IacprfNVVrw5JidC1ispGFuQ71tqQr-B6FMYaHA0jZPd0rz7YcQ0-gE6qiFGj5FuYhjRjH5WbkK3TWDMRzZbTNTF0NMnjN74qad5DmBwSlSjCxEpDJaCG3OMw9jjMMIu6JwcOegSXuznGXl_uH-7eypeXh-f725fCiNkMxZWMig1mKqyEhxD0TrHwUHTau5KtI45qLTUrtVVU2tuoQXOAWTdokFmxBm5mnNzh88pF1O9Twa7DgYMU1JlXa9KWVcrnqVylpoYUoro1Cb6HuJWcaZ28NVa_cJXO_hqhp-Nl_sfk-7R_tl-aWfBzSzA3PQrE1XJeBwMWh8zV2WD_-_HD-O-nbA</recordid><startdate>20230401</startdate><enddate>20230401</enddate><creator>Su, Yue</creator><creator>Cheng, Shasha</creator><creator>Ding, Yixin</creator><creator>Wang, Linge</creator><creator>Sun, Mingshuang</creator><creator>Man, Chaoxin</creator><creator>Zhang, Yu</creator><creator>Jiang, Yujun</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20230401</creationdate><title>A comparison of study on intestinal barrier protection of polysaccharides from Hericium erinaceus before and after fermentation</title><author>Su, Yue ; Cheng, Shasha ; Ding, Yixin ; Wang, Linge ; Sun, Mingshuang ; Man, Chaoxin ; Zhang, Yu ; Jiang, Yujun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c368t-d60a2bac55d6af0e39ff1afa89b1f2edf0fa5b6bf9b587b1da9a11aa679ece0c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Animals</topic><topic>Fermentation</topic><topic>Fermented</topic><topic>Gut microbiota</topic><topic>Intestinal barrier</topic><topic>Intestinal Mucosa - metabolism</topic><topic>Intestines</topic><topic>Mice</topic><topic>Polysaccharide</topic><topic>Polysaccharides - metabolism</topic><topic>Polysaccharides - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Su, Yue</creatorcontrib><creatorcontrib>Cheng, Shasha</creatorcontrib><creatorcontrib>Ding, Yixin</creatorcontrib><creatorcontrib>Wang, Linge</creatorcontrib><creatorcontrib>Sun, Mingshuang</creatorcontrib><creatorcontrib>Man, Chaoxin</creatorcontrib><creatorcontrib>Zhang, Yu</creatorcontrib><creatorcontrib>Jiang, Yujun</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of biological macromolecules</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Su, Yue</au><au>Cheng, Shasha</au><au>Ding, Yixin</au><au>Wang, Linge</au><au>Sun, Mingshuang</au><au>Man, Chaoxin</au><au>Zhang, Yu</au><au>Jiang, Yujun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A comparison of study on intestinal barrier protection of polysaccharides from Hericium erinaceus before and after fermentation</atitle><jtitle>International journal of biological macromolecules</jtitle><addtitle>Int J Biol Macromol</addtitle><date>2023-04-01</date><risdate>2023</risdate><volume>233</volume><spage>123558</spage><epage>123558</epage><pages>123558-123558</pages><artnum>123558</artnum><issn>0141-8130</issn><eissn>1879-0003</eissn><abstract>The intestinal barrier protects the host from harmful substances. This paper investigated two polysaccharides extracted from the Hericium erinaceus before and after fermentation (HEP and FHEP). The effects of two polysaccharides on the intestinal barrier were investigated in cell and mice models. The results showed that polysaccharides had a protective effect against acrylamide-induced injury in IEC-6 cell. Compared with HEP, FHEP significantly increased TEER and paracellular permeability (P < 0.05). Both polysaccharides the expression of alter tight junction (TJ) and mucin (MUC) as observed in cell Western Bolt (WB). Polysaccharides also enhance the intestinal barrier function in mice by improving cyclophosphamide induced cytokines level, TJ and MUC expression, and gut microbiota. The results showed that FHEP significantly increased IgA, IgG, and IgM levels while decreasing TNF-, IL-1, and IL-6 levels (P < 0.05). The immunohistochemical results showed that both polysaccharides significantly increased the expression of occludin, ZO-1, ZO-2, claudin-3, claudin-4, MUC2 and decreased claudin-2. In parallel, polysaccharides could alter the composition of the gut microbiota, indicating that increased in Bacteriodetes, Firmicutes and decreased in Klebsiella and Shigella. This work provides important views on the protective effect of fermented polysaccharides on the intestinal barrier, and provides a potential mechanism for the beneficial health properties of these biomacromolecules.
[Display omitted]
•FHEP had a more significant protective effect in vitro and in vivo.•FHEP could better prevent the acrylamide induced damage of intestinal barrier.•HEP and FHEP could maintain the intestinal immune barrier of mice.•HEP and FHEP were found to increase the abundance of beneficial bacteria.•HEP and FHEP could improve the intestinal barrier function.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>36746300</pmid><doi>10.1016/j.ijbiomac.2023.123558</doi><tpages>1</tpages></addata></record> |
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| subjects | Animals Fermentation Fermented Gut microbiota Intestinal barrier Intestinal Mucosa - metabolism Intestines Mice Polysaccharide Polysaccharides - metabolism Polysaccharides - pharmacology |
| title | A comparison of study on intestinal barrier protection of polysaccharides from Hericium erinaceus before and after fermentation |
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