Rapid and sensitive biosensor for Salmonella

The rapid and sensitive detection of Salmonella typhymurium based on the use of a polyclonal antibody immobilized by the Langmuir–Blodgett method on the surface of a quartz crystal acoustic wave device was demonstrated. The binding of bacteria to the surface changed the crystal resonance parameters;...

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Veröffentlicht in:	Biosensors & bioelectronics 2000-06, Vol.15 (3), p.135-141
Hauptverfasser:	Pathirana, S.T, Barbaree, J, Chin, B.A, Hartell, M.G, Neely, W.C, Vodyanoy, V
Format:	Artikel
Sprache:	eng
Schlagworte:	Acoustic wave device Animals Antibodies, Bacterial Antigen–antibody monolayers Bacteriological Techniques Biological and medical sciences Biosensing Techniques - methods Biosensing Techniques - statistics & numerical data Biosensors Biotechnology Food Microbiology Fundamental and applied biological sciences. Psychology Humans Langmuir–Blodgett immobilization Methods. Procedures. Technologies Salmonella typhimurium Salmonella typhimurium - immunology Salmonella typhimurium - isolation & purification Sensitivity and Specificity Sensor Time Factors Various methods and equipments
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Zusammenfassung:	The rapid and sensitive detection of Salmonella typhymurium based on the use of a polyclonal antibody immobilized by the Langmuir–Blodgett method on the surface of a quartz crystal acoustic wave device was demonstrated. The binding of bacteria to the surface changed the crystal resonance parameters; these were quantified by the output voltage of the sensor instrumentation. The sensor had a lower detection limit of a few hundred cells/ml, and a response time of
ISSN:	0956-5663 1873-4235
DOI:	10.1016/S0956-5663(00)00067-1