miR-34a regulates silent synapse and synaptic plasticity in mature hippocampus

AMPAR-lacking silent synapses are prevailed and essential for synaptic refinement and synaptic plasticity in developing brains. In mature brain, they are sparse but could be induced under several pathological conditions. How they are regulated molecularly is far from clear. miR-34a is a highly conserved and brain-enriched microRNA with age-dependent upregulated expression profile. Its neuronal function in mature brain remains to be revealed. Here by analyzing synaptic properties of the heterozygous miR-34a knock out mice (34a_ht), we have discovered that mature but not juvenile 34a_ht mice have more silent synapses in the hippocampus accompanied with enhanced synaptic NMDAR but not AMPAR function and increased spine density. As a result, 34a_ht mice display enhanced long-term potentiation (LTP) in the Schaffer collateral synapses and better spatial learning and memory. We further found that Creb1 is a direct target of miR-34a, whose upregulation and activation may mediate the silent synapse increment in 34a_ht mice. Hence, we reveal a novel physiological role of miR-34a in mature brains and provide a molecular mechanism underlying silent synapse regulation. [Display omitted] •Reducing miR-34a selectively enhanced NMDAR-mediated synaptic transmission in mature adult but not juvenile hippocampus.•Adult 34a_ht mice have more silent synapses in CA1 of the hippocampus.•miR-34a directly targets Creb1.•34a_ht mice have elevated CREB1 expression and activation that correlates with more silent synapses.•34a_ht mice express stronger LTP in the Schaffer collateral synapses and display better spatial learning and memory.