Different nitrogen fertilizer application in the field affects the morphology and structure of protein and starch in rice during cooking

[Display omitted] •More protein bodies adhere to the surface of amyloplasts under high nitrogen fertilizer.•Protein bodies slows down damage to the starch structure by cooking.•Protein bodies inhibits the distribution of protons during rice cooking.•Rice eating quality is related to protein and starch structure change during cooking. Nitrogen fertilization is one of the most important cultivation practices that affects the eating quality of rice. During the cooking process, nitrogen fertilizer application in the field changed the structure of protein and starch during cooking, which eventually reduced the rice eating quality. However, the morphology and structure of rice during cooking under high nitrogen fertilizer application in the field have not been explored. The relationship between the morphological and structural changes of rice protein and starch during cooking and the rice eating quality has not been studied. In this study, we conducted field trials at two nitrogen fertilizer levels (0 N and 350 N), and the rice was cooked after harvest. Our results showed that the peak viscosity of rice flour was 3326 cp and 2453 cp at 0 N and 350 N, respectively, and the peak viscosity of rice starch was 3424 cp and 3378 cp, respectively. Rice proteins played an important role in the starch gelatinization properties and thermodynamic properties. High nitrogen fertilizer application increased the protein content of rice from 5.97 % to 11.32 %, and more protein bodies adhered to the surface of amyloplasts eventually inhibiting starch gelatinization. The rice proteins could bind to amylose–lipid complexes during cooking, promoting the formation of V-type diffraction peaks. What is more, under high nitrogen fertilizer, rice protein had more β-sheets, which slowed the entry of water into the interior of starch molecules and prevented the destruction of the short-range ordered structure of starch. Our study provides the possibility to further improve the eating quality of rice under nitrogen fertilizer treatment.