Cytotoxic steroidal glycosides from the rhizomes of Paris polyphylla var. yunnanensis

Paris polyphylla var. yunnanensis (Franch.) Hand.-Mazz. (Melanthiaceae), an important specie of the genus Paris, has long been in a traditional Chinese medicine (TCM) for a long time. This study aimed to isolate and identify the structures of bioactive saponins from the rhizomes of P. polyphylla var. yunnanensis and evaluate their cytotoxicity against BxPC-3, HepG2, U373 and SGC-7901 carcinoma cell lines. Seven previously undescribed and seven known saponins were identified, and Paris saponins VII (PSVII) showed significant cytotoxicity against the BxPC-3 cell line with IC50 values of 3.59 μM. Furthermore, flow cytometry, transmission electron microscopy and western-bolt analysis revealed that PSVII inhibited the proliferation of BxPC-3 cells and might be involved in inducing apoptosis and pyroptosis by activating caspase-3, -7 and caspase-1, respectively. [Display omitted] •Seven undescribed saponins were isolated from the rhizomes of Paris polyphylla var. yunnnanensis.•The cytotoxicities of all isolated compounds against several cancer cell lines were evaluated.•Paris saponin VII inhibits the proliferation of BxPC-3 cells by inducing apoptosis and pyroptosis.