Asymmetric and symmetric dimethylarginine gingival crevicular fluid levels in periodontitis

Objective This study aimed to evaluate the level of ADMA (asymmetric dimethylarginine), SDMA (symmetric dimethylarginine), and IL‐1β (Interleukin‐1β) in gingival crevicular fluid (GCF) from periodontitis patients and control subjects. Background ADMA and SDMA are potentially hazardous non‐proteinogenic amino acids that limit nitric oxide (NO) synthesis and have many functions in various human disorders. ADMA causes a structural change in nitric oxide synthase, while SDMA blocks arginine cell uptake. Increased plasma ADMA has been widely recognized as a “trigger” initiating impaired NO bioavailability and vascular dysfunction, which ultimately leads to oxidative stress. Methods Twenty‐five patients with periodontitis (P) (Stage III, Grade C, n = 25) and 20 control (C) subjects were included in the study. The IL‐1β level of GCF was measured by enzyme immunoassay (ELISA) and ADMA and SDMA by liquid chromatography‐mass spectrometry (LC–MS/MS). Results Periodontitis patients had higher clinical parameters than controls (p