Testosterone replacement relieves ligature-induced periodontitis by mitigating inflammation, increasing pro-resolving markers and promoting angiogenesis in rats: A preclinical study

This study aimed to evaluate the inflammatory profile as well as the resolution of inflammation in a ligature-induced periodontal inflammation in rats with depletion and/or supraphysiological testosterone replacement. Sixty male rats (Holtzman) were used in the present study. Study groups were creat...

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Veröffentlicht in:Archives of oral biology 2023-02, Vol.146, p.105605-105605, Article 105605
Hauptverfasser: Pelegrin, Álvaro Formoso, de Paiva Gonçalves, Vinícius, Carvalho, Jhonatan de Souza, Spolidorio, Denise Madalena Palomari, Spolidorio, Luís Carlos
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Sprache:eng
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Zusammenfassung:This study aimed to evaluate the inflammatory profile as well as the resolution of inflammation in a ligature-induced periodontal inflammation in rats with depletion and/or supraphysiological testosterone replacement. Sixty male rats (Holtzman) were used in the present study. Study groups were created as following: (1) Sham (no testicle removal); (2) Orchiectomy (OCX), 3) OCX + Testosterone (OCX + T); (4) Sham + Ligature (SH + L); (5) OCX+L; and 6) OCX + T + L. The surgeries were performed on day 1, and testosterone was administered weekly since day 1. On day 15, a cotton ligature was placed around the lower first molars and maintained for 15 days. Morphological changes in periodontal tissues were determined by histopathological analysis. Immunohistochemistry (factor VIII) and immunoenzymatic assay were performed to evaluate angiogenesis process and (pro- and anti-) inflammatory markers, respectively. Ligature promoted a marked inflammatory gingival infiltrate and bone loss (P 
ISSN:0003-9969
1879-1506
DOI:10.1016/j.archoralbio.2022.105605