Robust and Reliable Fabrication of Gelatin Films Containing Micropatterned Opal Structures via Evaporative Deposition and Thermal Gelation

Biopolymeric hydrogel materials containing tunable optical properties such as micropatterned artificial opal structures hold significant potential in various applications. Despite recent advances in fabrication techniques, simple, reliable, and tunable production of stimuli-responsive micropatterned opal hydrogels under mild conditions remains challenging. We report a simple micromolding-based evaporative deposition–thermal gelation technique for gelatin films that capture uniform opal micropatterns, aided by a potent aminopolysaccharide chitosan (CS) that provides binding affinity and structural stability. Our results show reliable, tunable, and high-fidelity fabrication of gelatin hydrogel films containing CS-opal micropatterns, while the as-prepared films show responsiveness to pH, ionic strength, and water content indicating a robust nature. Uniform CS-opal microparticles can also be readily prepared via removal of the gelatin through various simple routes, illustrating the crucial roles of CS and gelatin. We envision that this robust, reliable, and simple evaporative deposition–thermal gelation technique can be readily extended to prepare responsive biopolymeric materials for various applications.