Matrine-producing endophytic fungus Galactomyces candidum TRP-7: screening, identification, and fermentation conditions optimization for Matrine production
Matrine (MA) is an alkaloid extracted from the root of genus Sophora with various pharmacological activities. Production of MA by endophytic fungi offers an alternative challenge to reduce the massive consumption to meet the increasing demand of MA. In the current study, the positive strains with MA...
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Veröffentlicht in: | Biotechnology letters 2023-02, Vol.45 (2), p.209-223 |
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Sprache: | eng |
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Zusammenfassung: | Matrine (MA) is an alkaloid extracted from the root of genus
Sophora
with various pharmacological activities. Production of MA by endophytic fungi offers an alternative challenge to reduce the massive consumption to meet the increasing demand of MA. In the current study, the positive strains with MA producing ability were screened from endophytic fungal isolated from the root of
Sophora tonkinensis
Gagnep. Chromatographic analyses verified the identity of the produced MA. Among these fungi,
Galactomyces candidum
strain TRP-7 was the most valuable strain for MA production with the initial yield 8.26 mg L
−1
. The MA production was efficiently maximized up to 17.57 mg L
−1
of fermentation broth, after optimization of eight process parameters using Plackett–Burman and Box-Behnken designs. The statistical optimization resulted in a 1.127 times increase in MA production as compared to the initial yield of TRP-7. This is the first report to isolate endophytic fungi with MA-producing activity from
S. tonkinensis
Gagnep., and to identify an endophytic fungus
G. candidum
TRP-7 as a new promising start strain for a higher MA yield. |
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ISSN: | 0141-5492 1573-6776 |
DOI: | 10.1007/s10529-022-03331-1 |