Detection of interaction between an RNA aptamer and its target compound in living human cells using 2D in-cell NMR

We introduced an isotopically labeled RNA aptamer for HIV-1 Tat prepared by E. coli transcription into HeLa cells. We successfully recorded the first heteronuclear 2D in-cell NMR spectra, which makes it possible to study the interaction of the RNA aptamer with argininamide in living human cells with higher resolution. A labeled RNA aptamer prepared with E. coli was introduced into human cells. Aptamer interaction was investigated using 2D in-cell NMR.