Lipidomic analysis and triglyceride profiles of fish oil: Preparation through silica gel column and enzymatic treatment

[Display omitted] •Composition of lipid classes and TGs were determined of fish oil with high purity EPA/DHA.•Overall, 1010 lipids belonging to 6 categories and 38 lipid classes were identified.•Enzymic treatments had slight effect on fish oil lipid distribution.•Silica gel column could enrich the lipids species.•TG’s nature structure might limit to further improve EPA/DHA purity by silica gel column, but enzymic method was not restricted by this. A comprehensive study of lipidomic coupled with triglyceride profiles onto four fish oils was performed through ultra-performance liquid chromatography-electrospray ionization tandem mass spectrometry (UPLC-ESI-MS/MS). Overall, 1010 lipids belonging to 6 categories and 38 lipid classes were identified. Triglycerides (TGs) were the dominant component in four fish oils (40 %–99 % of total lipids), and glycerophospholipids (GPs) and sphingolipids (SLs) were another two major lipid categories in the fish oil (TG50) which prepared through silica gel column. These results revealed that enzymatic treatment has slight effect on lipid distribution but silica gel column could change the lipids composition. TGs composition of four fish oils were separated completely, and the most TG molecule in TG50 is TG(18:3_14:0_18:0), possessed 13.03 ± 5.07 % relative content, these results implied that silica gel column could protect the nature structure of TGs from destroying which may also limited to further improve eicosapentaenoic acid/docosahexaenoic acid (EPA/DHA) purity, but enzymic method was not restricted by this.