Establishment and evaluation of multiple adulteration detection of camellia oil by mixture design

•Mixture design is used to prepare representative multiple adulterated camellia oils.•Recursive SVM model by using representative oils was built for multiple adulteration detection.•Evaluation of OCPLS model was conducted by representative adulterated camellia oils.•The percentage of four cheaper oils was optimized to evaluate OCPLS model. Multiple adulteration is a common trick to mask adulteration detection methods. In this study, the representative multiple adulterated camellia oils were prepared according to the mixture design. Then, these representative oils were employed to build two-class classification models and validate one-class classification model combined with fatty acid profiles. The cross-validation results indicated that the recursive SVM model possessed higher classification accuracy (97.9%) than PLS-DA. In OCPLS model, the optimal percentage of RO, SO, CO and SUO was 2.8%, 0%, 7.2%, 0% respectively in adulterated camellia oil, which is the most similar to the authentic camellia oils. Further validation showed that five adulterated oils with the optimal percentage could be correctly identified, indicating that the OCPLS model could identify multiple adulterated oils with these four cheaper oils. Moreover, this study serves as a reference for one class classification model evaluation and a solution for multiple adulteration detection of other foods.