The Evaluation of Different Bacteriocinogenic Bacillus spp. with Activity Against Staphylococcus spp. and Their Beneficial and/or Hazardous Properties
The aim of this project was to screen for bacteriocinogenic Bacillus strains with activity versus Staphylococcus spp. with future application in formulation of pharmaceutical antimicrobial preparations. Putative bacteriocinogenic strains, isolated and pre-identified as Bacillus spp. were selected fo...
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Veröffentlicht in: | Probiotics and antimicrobial proteins 2024-02, Vol.16 (1), p.35-52 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | The aim of this project was to screen for bacteriocinogenic
Bacillus
strains with activity versus
Staphylococcus
spp. with future application in formulation of pharmaceutical antimicrobial preparations. Putative bacteriocinogenic strains, isolated and pre-identified as
Bacillus
spp. were selected for future study and differentiated based on repPCR and identified as
Bacillus subtilis
for strains ST826CD and ST829CD,
Bacillus subtilis
subsp.
stercoris
for strain ST794CD,
Bacillus subtilis
subsp.
spizizenii
for strain ST824CD,
Bacillus velezensis
for strain ST796CD, and
Bacillus tequilensis
for strain ST790CD. Selected strains were evaluated regarding their safety/virulence, beneficial properties, and potential production of antimicrobials based on biomolecular and physiological approves. Expressed bacteriocins were characterized regarding their proteinaceous nature, stability at different levels of pH, temperatures, and the presence of common chemicals applied in bacterial cultivation and bacteriocin purification. Dynamic of bacterial growth, acidification, and cumulation of produced bacteriocins and some aspects of the bacteriocins mode of action were evaluated. Based on obtained results, isolation and application of expressed antimicrobials can be realistic scenario for treatment of some staphylococcal associated infections. Appropriate biotechnological approaches need to be developed for cost effective production, isolation, and purification of expressed antimicrobials by studied
Bacillus
strains. |
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ISSN: | 1867-1306 1867-1314 |
DOI: | 10.1007/s12602-022-10017-7 |