Effect of phytic acid on dentinal collagen solubilization and its binding and debinding potentials to dentin

•Phytic acid effect on collagen solubilization was studied and compared to phosphoric acid.•Overall hydroxyproline release induced by phytic acid was lower compared to phosphoric acid.•Phytic acid has the ability to bind to mineralized and demineralized dentin.•Phytic acid debound from mineralized dentin mostly by the action of urea.•Phytic acid debound from demineralized dentin mostly by the action of ethanol. To study phytic acid (IP6) effect on collagen solubilization by assessing hydroxyproline (HYP) release, evaluate its binding to demineralized (DD) and mineralized dentin (MD) and determine the effect of different media on debinding of IP6. Demineralized dentin beams were incubated in 1%, 2% or 3% IP6 and HYP release was evaluated at 1 or 3 weeks and compared to those obtained in untreated control or phosphoric acid (PA)-treated beams. DD or MD powder was treated with 1%, 2% or 3% IP6 and the decrease in IP6 amount was quantitated by ultraviolet–visible spectroscopy. IP6-treated samples were re-suspended in distilled water, ethanol, urea or sodium chloride and the amount of IP6 displaced was determined. At 1 week, the control group and IP6 showed lower HYP release when compared to PA (P < 0.05). There was no difference among PA, IP6 and control at 3 weeks (P = 0.22). IP6 binding was concentration dependent. 1% IP6 had higher binding potential with MD compared to DD while 2% IP6 showed the opposite result (P