A Highly Compatible Phototrophic Community for Carbon‐Negative Biosynthesis

CO2 sequestration engineering is promising for carbon‐negative biosynthesis, and artificial communities can solve more complex problems than monocultures. However, obtaining an ideal photosynthetic community is still a great challenge. Herein, we describe the development of a highly compatible photosynthetic community (HCPC) by integrating a sucrose‐producing CO2 sequestration module and a super‐coupled module. The cyanobacteria CO2 sequestration module was obtained using stepwise metabolic engineering and then coupled with the efficient sucrose utilization module Vibrio natriegens. Integrated omics analysis indicated that enhanced photosynthetic electron transport and extracellular vesicles promote intercellular communication. Additionally, the HCPC was used to channel CO2 into valuable chemicals, enabling the overall release of −22.27 to −606.59 kgCO2e kg−1 in the end products. This novel light‐driven community could facilitate circular economic implementation in the future. A highly compatible phototrophic community (HCPC) was developed through the integration of a CO2 sequestration module and a super‐coupled module, V. natriegens. Based on the HCPC platform, various chemicals could be directly synthesized from CO2, enabling the overall release of −22.27 to −606.59 kgCO2e kg−1 in the end products. This study provides design guidance for photosynthetic communities and new opportunities for carbon‐negative production.