The Dietary Supplementation of Copper and Zinc Nanoparticles Improves Health Condition of Young Dairy Calves by Reducing the Incidence of Diarrhoea and Boosting Immune Function and Antioxidant Activity

This study was conducted to evaluate the effect of nano copper (nano Cu) and nano zinc (nano Zn) supplementation on the biomarkers of immunity and antioxidant and health status attributes in young dairy calves. Twenty-four young cattle calves were randomly assigned into four groups (6 calves per group) on a body weight and age basis for a period of 120 days. The feeding regimen was the same in all the groups except that these were supplemented with 0.0 mg nano Cu and nano Zn (control), 10 mg nano Cu ( nano Cu 10 ), 32 mg nano Zn (nanoZn 32 ), and a combination of nano Cu and nano Zn ( nano Cu 10 + nano Zn 32 ) per kg dry matter (DM) basis in four respective groups. Supplementation of nano Cu along with nano Zn improves immune response which was evidenced from higher immunoglobulin G (IgG), immunoglobulin M (IgM), immunoglobulin A (IgA), total immunoglobulin (TIg), and Zn sulphate turbidity (ZST) units and lower plasma concentrations of tumour necrosis factor-α (TNF-α) and cortisol in the nanoCu10 + nanoZn32 group. There was no effect of treatment on the plasma concentrations of immunoglobulin E (IgE) and interferon-gamma (IFN-γ). Antioxidant status was also better in the nanoCu10 + nanoZn32 group as evidenced by lower concentrations of malondialdehyde (MDA) and higher activity of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), ceruloplasmin (Cp), and total antioxidant status (TAS). However, treatment did not exert any effect on catalase (CAT) activity. Although the nano Cu or nano Zn supplementation, either alone or in combination, did not exert any effect on growth performance or body condition score (BCS), the frequency of diarrhoea and incidence of diarrhoea were lower, while faecal consistency score (FCS) and attitude score were better in the nano Cu 10 + nano Zn 32 groups. In the control group, one calf was found affected with joint illness and two calves were found affected with navel illness. During the experimental period, none of the calves in all four groups were found to be affected by pneumonia. The findings of this study revealed that dietary supplementation of nano Cu in combination with nano Zn improved the health status of young dairy calves by improving immunity and antioxidant status.