Alteration of hippocampal Egr3, GABA A receptors, Il-1β, Il6 and Ccl3 expression in audiogenic tremor mice after seizure

•Hippocampal proinflammatory cytokines and GABA A receptors were altered after a seizure.•Overexpression of Ccl3 mRNA was observed in the hippocampus of naive tremor mice.•Hippocampal Il-1β and Ccl3 expression were downregulated in stimulated tremor mice.•Low expression of Egr3, Gabra1, and Gabra4 mRNA were detected in naive tremor mice.•Egr3, Gabra1, and Gabra4 expression increased in the hippocampus of stimulated mice. Neuroinflammation plays a protective role in the brain; however, in neurological diseases such as epilepsy, overactivated neuroinflammation, along with overexpression of inflammatory mediators, can cause neuronal tissue damage, which can trigger seizures due to loss of ionic or neurotransmitter homeostasis. Therefore, we aimed to evaluate mRNA expression levels of proinflammatory cytokines, early growth response factor 3 (Egr3), and GABA A receptors in the hippocampus of naive audiogenic mutant tremor mice, and stimulated tremor mice after a seizure. Gene expression of Il-1β, Il-6, Tnf-α, Ccl2, Ccl3, Egr3, Gabra1, and Gabra4 from hippocampal samples of naive and stimulated tremor mice were measured by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Relative to resistant mice, Ccl3 gene expression was increased and Il6 was decreased in the hippocampus of naïve tremor mice. Thirty minutes after a seizure, Ccl3 and Il-1β mRNA expression were decreased (p