Quantum Cascade Laser Based Infrared Spectroscopy: A New Paradigm for Protein Secondary Structure Measurement

Mid-infrared spectroscopy is one of the major analytical techniques employed for measurements of protein structure in solution. Traditional Fourier Transform-Infrared (FT-IR) measurement is limited by its blackbody light source that is inherently spatially incoherent and has low optical power output...

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Veröffentlicht in:	Pharmaceutical research 2023-06, Vol.40 (6), p.1507-1517
Hauptverfasser:	Jin, Chunguang, Patel, Amrish, Peters, Jeremy, Hodawadekar, Santosh, Kalyanaraman, Ravi
Format:	Artikel
Sprache:	eng
Schlagworte:	Albumin Amides Analysis Biochemistry Biomedical and Life Sciences Biomedical Engineering and Bioengineering Biomedicine Bovine serum albumin Infrared spectroscopy Laser radiation Lasers Lasers, Semiconductor Light sources Measurement Medical Law Original Research Article Pharmacology/Toxicology Pharmacy Protein structure Proteins Secondary structure Serum Albumin, Bovine Spectrophotometry, Infrared - methods Spectroscopy, Fourier Transform Infrared - methods Spectrum analysis Water - chemistry
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Zusammenfassung:	Mid-infrared spectroscopy is one of the major analytical techniques employed for measurements of protein structure in solution. Traditional Fourier Transform-Infrared (FT-IR) measurement is limited by its blackbody light source that is inherently spatially incoherent and has low optical power output. This limitation is pronounced when working with proteins in aqueous solutions. Strong absorbance of water in protein amide I region 1600–1700 cm −1 restricts light path length to
ISSN:	0724-8741 1573-904X
DOI:	10.1007/s11095-022-03422-8