An integrated transcriptomic and metabolomic analysis for changes in rose plant induced by rose powdery mildew and exogenous salicylic acid

We explored the transcriptomic and metabolomic changes in Rosa chinensis after the infection with Podosphaera pannosa and after the treatment with exogenous salicylic acid (SA), separately. The rose responses to the mildew-infection were clearly similar to the responses to the SA-treatment. Based on the combined omics analysis, after the induction by both P. pannosa and SA, R. chinensis responded consistently by MAPK cascades, plant-pathogen interaction pathway activation, and resistance (R) genes expression, and further, triterpenoid biosynthesis, glutathione metabolism, and linoleic acid metabolism were significantly enriched when compared with the control. The levels of the triterpenoids with the largest fold change values were significantly up-regulated such as dehydro (11,12) ursolic acid lactone and maslinic acid, suggesting that these pathways and metabolites were involved in the resistance to P. pannosa. The contents of salicylic acid beta-D-glucoside, methyl salicylate, and methyl jasmonate increased significantly resulting from both P. pannosa-infection and exogenous SA-treatment. •Exogenous salicylic acid (SA) was used to treat rose plants and the effect of SA on roses were very similar to the effect of the mildew.•We first found that a triterpenoid, dehydro (11,12) ursolic acid lactone, was involved in response to the infection.•We found that several genes were involved in the indirect host plant-mediated interactions between fungi and insects.