Hydrogen Production by CO2 Deprived Photoautotrophic Chlamydomonas reinhardtii Cultures

Light-dependent hydrogen production by microalgae attracts attention of researchers because of the potential practical application. It is generally recognized that Calvin–Benson–Bassham cycle competes with hydrogen production process for electrons, and substrate (CO 2 ) limitation of the cycle can increase hydrogen production rate. Furthermore, photosystem II is not destroyed by CO 2 deficiency. We studied photoautotrophic cultures of Chlamydomonas reimhardtii under CO 2 deficiency. Under the flow of air with removed CO 2 the cultures reached stationary phase of growth and the photosystem II was downregulated due to overreduction of plastoquinone pool followed by degradation of the entire photosynthetic machinery. Under the Ar flow in the absence of CO 2 the cultures were brought to microaerobic conditions producing small amounts of hydrogen (5 ml H 2 day –1 liter –1 culture). Similar to the case of incubation under air atmosphere, prolonged incubation of cultures under microaerobic conditions resulted in down-regulation of photosystem II due to overreduction of plastoquinone pool with following degradation of whole photosynthetic machinery. Following removal of CO 2 , transfer of cultures into dark anaerobic conditions (2.5 h), and illumination with low-intensity light resulted in the cultures producing H 2 with high initial rate. Total microalgal hydrogen production under these conditions was 56 ml H 2 liter –1 culture. Thus, the CO 2 -deprived photoautotrophic cultures produce hydrogen. Hydrogen production was limited by the toxic effect of oxygen on hydrogenase but not by the Calvin–Benson–Bassham cycle competition with hydrogen production process.