Bioaugmentation performances with a powerful strain for nitrogen removal without N2O accumulation

N2O is regarded as an inevitable intermediate during nitrogen removal, especially for wastewater treatment plants where good operating conditions would be required to mitigate N2O releasing, which generally causes a high treatment cost. In this study, a novel bacterium capable of removing nitrogen without N2O accumulation was isolated and identified as Citrobacter freundii XY-1. The nitrogen removal characteristics, nitrogen removal pathway, bioaugmentation in different reactors as well as microbial diversity were investigated. Results showed that 99.42% of NH+ 4-N and 95% of total organic carbon could be removed within 48 h with the corresponding removal rates being 4.03 mg/(L·h) and 39.42 mg/(L·h), respectively. It was inferred that traditional denitrification and N2O generation do not exist in the pathway of removing nitrogen by XY-1 based on isotope analysis and functional genes detection. Bioaugmentations of XY-1 in both sequencing batch reactor and biological aerated filter significantly promoted the performances of nitrogen removal. The microbial diversity indicated that the relative abundance of strain XY-1 ranged from 45% to 66%, predominating throughout the running period. Overall, XY-1 could become an incredibly important candidate for the upgrading of wastewater treatment plants. [Display omitted] •A novel bacterium was isolated and identified as Citrobacter freundii XY-1.•99.42% of NH+-N was removed within 48 h in batch experiments.•Traditional denitrification and N2O generation does not exist in XY-1.•Bioaugmentations of XY-1 notably promoted the performances of nitrogen removal.•XY-1 predominated throughout the running period.