Functional characterization of BdCIPK31 in plant response to potassium deficiency stress

Potassium (K) is one of the most essential macronutrients for plants. However, K+ is deficient in some cultivated soils. Hence, improving the efficiencies of K+ uptake and utilization is important for agricultural production. Ca2+ signaling pathways play an important role in regulation of K+ acquisition. In the present study, BdCIPK31, a Calcineurin B-like protein interacting protein kinase (CIPK) from Brachypodium distachyon, was found to be a potential positive regulator in plant response to low K+ stress. The expression of BdCIPK31 was responsive to K+-deficiency, and overexpression of BdCIPK31 conferred enhanced tolerance to low K+ stress in transgenic tobaccos. Furthermore, BdCIPK31 was demonstrated to promote the K+ uptake in root, and could maintain normal root growth under K+-deficiency conditions. Additionally, BdCIPK31 functioned in scavenging excess reactive oxygen species (ROS), reduced oxidative damage caused by low K+ stress. Collectively, our study indicates that BdCIPK31 is a vital regulatory component in K+-acquisition system in plants. •A new multi-function regulator in plant K+ utilization has been discovered.•BdCIPK31 enhances root K+ uptake, but doesn't affect root-shoot K+ transport.•BdCIPK31 promotes sugar transport under low K+ stress, contributes to root growth.•BdCIPK31 enhances ROS-scavenging, reduces oxidative damage caused by K-starving.