Structure characterization and anti-fatigue activity of an acidic polysaccharide from Panax ginseng C. A. Meyer

Panax ginseng C. A. Meyer is a traditional Chinese herbal medicine, which has been used in China for more than 2000 years. Its traditional effect of “invigorating vitality” is mainly reflected in anti-fatigue. However, due to the difficulty of identification of polysaccharide structure, there are few reports on homogeneous ginseng polysaccharide, and the molecular mechanism of its anti-fatigue effect remains to be further explored. In order to find the homogenous ginseng polysaccharide with the most anti-fatigue effect, this study is for the first time extracted, isolated and structurally identified polysaccharide monomer from Mountain Cultivated Ginseng (MCG). Then the anti-fatigue activity and molecular mechanism were studied. The structure of ginseng acidic polysaccharide APS-1 prepared by high performance gel permeation chromatography (HPGPC) was determined by acid hydrolysis/HPLC, methylation/GC-MS and NMR analysis. Anti-fatigue effect was evaluated by exhaustive swimming model, and AMPK axis-related proteins were detected by Western blot. APS-1 significantly prolonged fatigue tolerance time, alleviated accumulation of BLA, LDH and BUN, increased activities of SOD and CAT, alleviated oxidative damage caused by MDA, increased activity of CK, regulated glycolysis, and alleviated muscle fiber contraction. The expressions of LKB1, p-AMPK, PGC-1α and Glut4 in muscle were significantly up-regulated. The anti-fatigue effect of APS-1 was significantly, and the molecular mechanism may be related to the activation of AMPK axis signaling pathway to improve glucose uptake and mitochondrial function. [Display omitted] •The homogenous polysaccharide APS-1 was isolated and identified from Panax ginseng.•APS-1 regulates metabolite accumulation, oxidative damage and energy metabolism.•The anti-fatigue effect of APS-1 is related to AMPK axis signaling pathway.