The role of the endocannabinoid system in tooth eruption: An ex vivo study

The aim was to characterise the endocannabinoid system (ECS) in the dental pulp of teeth at different stages of eruption. Pulp of: erupted premolars (EPM), third molars in pre‐eruptive (PThM), intraosseous (IThM) and eruptive stages (EThM) ( n = 12 each group) were used. Messenger RNA expression of components of the ECS as cannabinoid receptors (CBr1 and CBr2), and anandamide synthetizing (NAPE–PLD) and degradation (FAAH) enzymes were measured by RT–PCR. Data were analysed using Student's t ‐test for comparisons between two groups and one‐way analysis of variance and Tukey's post‐test for multiple comparisons (statistical significance: p