Longitudinal surveillance of Cryptosporidium spp. in broiler chickens in Xinjiang, northwest China: genetic diversity of Cryptosporidium meleagridis subtypes
Cryptosporidium spp. are common enteric parasites in humans and animals. Herein, 175 faecal specimens were collected from a broiler farm in Xinjiang, China, including seven repeated samplings at 10-day intervals of broilers aged 10 to 70 days. Cryptosporidium was detected and identified by PCR–RFLP...
Gespeichert in:
Veröffentlicht in: | Parasitology research (1987) 2022-12, Vol.121 (12), p.3589-3595 |
---|---|
Hauptverfasser: | , , , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | Cryptosporidium
spp. are common enteric parasites in humans and animals. Herein, 175 faecal specimens were collected from a broiler farm in Xinjiang, China, including seven repeated samplings at 10-day intervals of broilers aged 10 to 70 days.
Cryptosporidium
was detected and identified by PCR–RFLP analysis. The overall infection rate of
Cryptosporidium
in broilers was 23.4% (41/175), with the highest infection rate of 48.0% (12/25) at 40 days of age, and no infection was detected at 10 days of age. Two
Cryptosporidium
species were confirmed, namely,
C. baileyi
(3.4%, 6/175) and
C. meleagridis
(20%, 35/175). In total, 21 of 35
C. meleagridis
isolates were successfully subtyped based on the
gp60
gene, and one known subtype, IIIgA22G3R1 (
n
= 1), and three novel subtypes, IIIbA25G1R1 (
n
= 10), IIIgA24G3R1 (
n
= 9) and IIIgA25G2R1 (
n
= 1), were identified. Our findings highlight the genetic diversity of
C. meleagridis
in Xinjiang and the potential endemic characteristics of the subtypes. |
---|---|
ISSN: | 0932-0113 1432-1955 |
DOI: | 10.1007/s00436-022-07683-5 |