Nitrogen budgets of a lower subtropical forest as affected by 6 years of over-canopy and understory nitrogen additions

Although tropical and subtropical regions have replaced temperate regions as the global-change hotspots for increased atmosphere nitrogen (N) deposition, whether the regional forests reach N saturation is still unclear. Understory or floor N addition has been commonly used in N-deposition studies, but the results of such studies have recently been challenged because they fail to account for canopy interception, assimilation, and leaching processes. Here, we conducted a field experiment to quantify the effects of over-canopy and understory N addition on N budgets in a lower subtropical monsoon evergreen broadleaved (LSMEB) forest. We found that the LSMEB forest was not N saturated after receiving additional N at 25 and 50 kg ha−1 yr−1 for 6 years. Plants were able to absorb the added N by increasing the N concentrations in their organs, with 120–412 % increasing trend of plant N pools under N-addition treatments. Canopy absorption of N resulting from over-canopy N addition led to increases in N concentrations in tree organs but not to increases in tree biomass. Understory N addition could underestimate the effects of N deposition in forests due to neglecting canopy N interception and canopy effects on N redistribution. Additional experiments using over-canopy N addition are needed to assess the true effects of N deposition on different forest ecosystems in different climate zones. [Display omitted] •The lower subtropical forest was not N-saturated after 6 years of N addition.•Plant community absorbed the added N by increasing the organ N concentrations, while soil N pool kept stable.•Understory N addition underestimated the effects of N deposition in forests.