Dysregulation of Long Noncoding RNA NEAT1/miR-199a-5/BiP Axis in Patients with Diabetic Neuropathy
Abstract Objective Diabetic neuropathy (DN) is a type of nerve damage and the most common complication of diabetes. Regarding the association between endoplasmic reticulum (ER) stress with the pathogenesis of neuropathy, this study aims to examine binding immunoglobulin protein (BiP) gene expression...
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Veröffentlicht in: | Laboratory medicine 2023-03, Vol.54 (2), p.160-165 |
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creator | Hassani, Seyedeh Sara Karamali, Negin Rajabinejad, Misagh Ashjari, Donya Afshar Hezarkhani, Leila Gorgin Karaji, Ali Salari, Farhad Rezaiemanesh, Alireza |
description | Abstract
Objective
Diabetic neuropathy (DN) is a type of nerve damage and the most common complication of diabetes. Regarding the association between endoplasmic reticulum (ER) stress with the pathogenesis of neuropathy, this study aims to examine binding immunoglobulin protein (BiP) gene expression and long noncoding RNA nuclear enriched abundant transcript 1 (NEAT1), miR-199a-5 as its regulator in the peripheral blood of DN patients compared to diabetic patients without neuropathy.
Methods
Peripheral blood samples were obtained from DN (n = 20) patients and diabetic patients without neuropathy (non-DN) (n = 20). After RNA extraction from peripheral blood mononuclear cells, reverse transcription-quantitative polymerase chain reaction was performed to evaluate RNA expression.
Results
The results showed that the expression level of NEAT1 and BiP genes in the DN group increased significantly compared to the non-DN group. Also, the expression level of miR-199a-5p in the DN group was significantly downregulated.
Conclusion
As a result, the axis of NEAT1, miR-199a-5p, and BiP may have a role in the DN pathogenesis. |
doi_str_mv | 10.1093/labmed/lmac082 |
format | Article |
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Objective
Diabetic neuropathy (DN) is a type of nerve damage and the most common complication of diabetes. Regarding the association between endoplasmic reticulum (ER) stress with the pathogenesis of neuropathy, this study aims to examine binding immunoglobulin protein (BiP) gene expression and long noncoding RNA nuclear enriched abundant transcript 1 (NEAT1), miR-199a-5 as its regulator in the peripheral blood of DN patients compared to diabetic patients without neuropathy.
Methods
Peripheral blood samples were obtained from DN (n = 20) patients and diabetic patients without neuropathy (non-DN) (n = 20). After RNA extraction from peripheral blood mononuclear cells, reverse transcription-quantitative polymerase chain reaction was performed to evaluate RNA expression.
Results
The results showed that the expression level of NEAT1 and BiP genes in the DN group increased significantly compared to the non-DN group. Also, the expression level of miR-199a-5p in the DN group was significantly downregulated.
Conclusion
As a result, the axis of NEAT1, miR-199a-5p, and BiP may have a role in the DN pathogenesis.</description><identifier>ISSN: 0007-5027</identifier><identifier>EISSN: 1943-7730</identifier><identifier>DOI: 10.1093/labmed/lmac082</identifier><identifier>PMID: 36166353</identifier><language>eng</language><publisher>US: Oxford University Press</publisher><subject>Antisense RNA ; Care and treatment ; Diabetes Mellitus ; Diabetic neuropathies ; Diabetic Neuropathies - genetics ; Diabetics ; Genes ; Genetic transcription ; Humans ; Leukocytes, Mononuclear - metabolism ; MicroRNAs - genetics ; MicroRNAs - metabolism ; Protein binding ; RNA, Long Noncoding - genetics ; RNA, Long Noncoding - metabolism ; Type 2 diabetes</subject><ispartof>Laboratory medicine, 2023-03, Vol.54 (2), p.160-165</ispartof><rights>The Author(s) 2022. Published by Oxford University Press on behalf of American Society for Clinical Pathology. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com 2022</rights><rights>The Author(s) 2022. Published by Oxford University Press on behalf of American Society for Clinical Pathology. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.</rights><rights>COPYRIGHT 2023 Oxford University Press</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c441t-3b6f0c7481d0138aeba35146713254ccfc8dc35c4122eec1a3ec7bd8a5e256e83</citedby><cites>FETCH-LOGICAL-c441t-3b6f0c7481d0138aeba35146713254ccfc8dc35c4122eec1a3ec7bd8a5e256e83</cites><orcidid>0000-0003-2551-705X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1578,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36166353$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hassani, Seyedeh Sara</creatorcontrib><creatorcontrib>Karamali, Negin</creatorcontrib><creatorcontrib>Rajabinejad, Misagh</creatorcontrib><creatorcontrib>Ashjari, Donya</creatorcontrib><creatorcontrib>Afshar Hezarkhani, Leila</creatorcontrib><creatorcontrib>Gorgin Karaji, Ali</creatorcontrib><creatorcontrib>Salari, Farhad</creatorcontrib><creatorcontrib>Rezaiemanesh, Alireza</creatorcontrib><title>Dysregulation of Long Noncoding RNA NEAT1/miR-199a-5/BiP Axis in Patients with Diabetic Neuropathy</title><title>Laboratory medicine</title><addtitle>Lab Med</addtitle><description>Abstract
Objective
Diabetic neuropathy (DN) is a type of nerve damage and the most common complication of diabetes. Regarding the association between endoplasmic reticulum (ER) stress with the pathogenesis of neuropathy, this study aims to examine binding immunoglobulin protein (BiP) gene expression and long noncoding RNA nuclear enriched abundant transcript 1 (NEAT1), miR-199a-5 as its regulator in the peripheral blood of DN patients compared to diabetic patients without neuropathy.
Methods
Peripheral blood samples were obtained from DN (n = 20) patients and diabetic patients without neuropathy (non-DN) (n = 20). After RNA extraction from peripheral blood mononuclear cells, reverse transcription-quantitative polymerase chain reaction was performed to evaluate RNA expression.
Results
The results showed that the expression level of NEAT1 and BiP genes in the DN group increased significantly compared to the non-DN group. Also, the expression level of miR-199a-5p in the DN group was significantly downregulated.
Conclusion
As a result, the axis of NEAT1, miR-199a-5p, and BiP may have a role in the DN pathogenesis.</description><subject>Antisense RNA</subject><subject>Care and treatment</subject><subject>Diabetes Mellitus</subject><subject>Diabetic neuropathies</subject><subject>Diabetic Neuropathies - genetics</subject><subject>Diabetics</subject><subject>Genes</subject><subject>Genetic transcription</subject><subject>Humans</subject><subject>Leukocytes, Mononuclear - metabolism</subject><subject>MicroRNAs - genetics</subject><subject>MicroRNAs - metabolism</subject><subject>Protein binding</subject><subject>RNA, Long Noncoding - genetics</subject><subject>RNA, Long Noncoding - metabolism</subject><subject>Type 2 diabetes</subject><issn>0007-5027</issn><issn>1943-7730</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkbtv2zAQxokiQe0mXTsWBLIkg2xSfEmj8mgbwHAMI50JijrZLCTRFSW0_u9D1_YWoLjhHvh9h8N9CH2hZEZJzuaNKVuo5k1rLMnSD2hKc84SpRi5QFNCiEoESdUEfQrhV2x5LtOPaMIklZIJNkXl4z70sBkbMzjfYV_jhe82eOk76ysXq_WywMun4pXOW7dOaJ6bRMzv3QoXf13ArsOrqIRuCPiPG7b40ZkSBmfxEsbe78yw3V-jy9o0AT6f8hX6-e3p9eFHsnj5_vxQLBLLOR0SVsqaWMUzWhHKMgOlYYJyqShLBbe2tlllmbCcpimApYaBVWWVGQGpkJCxK3R73Lvr_e8RwqBbFyw0jenAj0GnimaScUIO6M0R3ZgGtOtqP_TGHnBdKCVFJpgUkZq9Q8WooHXWd1C7OH9PYHsf4ltrvetda_q9pkQf7NJHu_TJrij4ejp5_Dc_42d_InB3BPy4-9-yN0_Zna4</recordid><startdate>20230307</startdate><enddate>20230307</enddate><creator>Hassani, Seyedeh Sara</creator><creator>Karamali, Negin</creator><creator>Rajabinejad, Misagh</creator><creator>Ashjari, Donya</creator><creator>Afshar Hezarkhani, Leila</creator><creator>Gorgin Karaji, Ali</creator><creator>Salari, Farhad</creator><creator>Rezaiemanesh, Alireza</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-2551-705X</orcidid></search><sort><creationdate>20230307</creationdate><title>Dysregulation of Long Noncoding RNA NEAT1/miR-199a-5/BiP Axis in Patients with Diabetic Neuropathy</title><author>Hassani, Seyedeh Sara ; Karamali, Negin ; Rajabinejad, Misagh ; Ashjari, Donya ; Afshar Hezarkhani, Leila ; Gorgin Karaji, Ali ; Salari, Farhad ; Rezaiemanesh, Alireza</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c441t-3b6f0c7481d0138aeba35146713254ccfc8dc35c4122eec1a3ec7bd8a5e256e83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Antisense RNA</topic><topic>Care and treatment</topic><topic>Diabetes Mellitus</topic><topic>Diabetic neuropathies</topic><topic>Diabetic Neuropathies - genetics</topic><topic>Diabetics</topic><topic>Genes</topic><topic>Genetic transcription</topic><topic>Humans</topic><topic>Leukocytes, Mononuclear - metabolism</topic><topic>MicroRNAs - genetics</topic><topic>MicroRNAs - metabolism</topic><topic>Protein binding</topic><topic>RNA, Long Noncoding - genetics</topic><topic>RNA, Long Noncoding - metabolism</topic><topic>Type 2 diabetes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hassani, Seyedeh Sara</creatorcontrib><creatorcontrib>Karamali, Negin</creatorcontrib><creatorcontrib>Rajabinejad, Misagh</creatorcontrib><creatorcontrib>Ashjari, Donya</creatorcontrib><creatorcontrib>Afshar Hezarkhani, Leila</creatorcontrib><creatorcontrib>Gorgin Karaji, Ali</creatorcontrib><creatorcontrib>Salari, Farhad</creatorcontrib><creatorcontrib>Rezaiemanesh, Alireza</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Laboratory medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hassani, Seyedeh Sara</au><au>Karamali, Negin</au><au>Rajabinejad, Misagh</au><au>Ashjari, Donya</au><au>Afshar Hezarkhani, Leila</au><au>Gorgin Karaji, Ali</au><au>Salari, Farhad</au><au>Rezaiemanesh, Alireza</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dysregulation of Long Noncoding RNA NEAT1/miR-199a-5/BiP Axis in Patients with Diabetic Neuropathy</atitle><jtitle>Laboratory medicine</jtitle><addtitle>Lab Med</addtitle><date>2023-03-07</date><risdate>2023</risdate><volume>54</volume><issue>2</issue><spage>160</spage><epage>165</epage><pages>160-165</pages><issn>0007-5027</issn><eissn>1943-7730</eissn><abstract>Abstract
Objective
Diabetic neuropathy (DN) is a type of nerve damage and the most common complication of diabetes. Regarding the association between endoplasmic reticulum (ER) stress with the pathogenesis of neuropathy, this study aims to examine binding immunoglobulin protein (BiP) gene expression and long noncoding RNA nuclear enriched abundant transcript 1 (NEAT1), miR-199a-5 as its regulator in the peripheral blood of DN patients compared to diabetic patients without neuropathy.
Methods
Peripheral blood samples were obtained from DN (n = 20) patients and diabetic patients without neuropathy (non-DN) (n = 20). After RNA extraction from peripheral blood mononuclear cells, reverse transcription-quantitative polymerase chain reaction was performed to evaluate RNA expression.
Results
The results showed that the expression level of NEAT1 and BiP genes in the DN group increased significantly compared to the non-DN group. Also, the expression level of miR-199a-5p in the DN group was significantly downregulated.
Conclusion
As a result, the axis of NEAT1, miR-199a-5p, and BiP may have a role in the DN pathogenesis.</abstract><cop>US</cop><pub>Oxford University Press</pub><pmid>36166353</pmid><doi>10.1093/labmed/lmac082</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0003-2551-705X</orcidid></addata></record> |
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subjects | Antisense RNA Care and treatment Diabetes Mellitus Diabetic neuropathies Diabetic Neuropathies - genetics Diabetics Genes Genetic transcription Humans Leukocytes, Mononuclear - metabolism MicroRNAs - genetics MicroRNAs - metabolism Protein binding RNA, Long Noncoding - genetics RNA, Long Noncoding - metabolism Type 2 diabetes |
title | Dysregulation of Long Noncoding RNA NEAT1/miR-199a-5/BiP Axis in Patients with Diabetic Neuropathy |
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