Dysregulation of Long Noncoding RNA NEAT1/miR-199a-5/BiP Axis in Patients with Diabetic Neuropathy

Abstract Objective Diabetic neuropathy (DN) is a type of nerve damage and the most common complication of diabetes. Regarding the association between endoplasmic reticulum (ER) stress with the pathogenesis of neuropathy, this study aims to examine binding immunoglobulin protein (BiP) gene expression...

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Veröffentlicht in:Laboratory medicine 2023-03, Vol.54 (2), p.160-165
Hauptverfasser: Hassani, Seyedeh Sara, Karamali, Negin, Rajabinejad, Misagh, Ashjari, Donya, Afshar Hezarkhani, Leila, Gorgin Karaji, Ali, Salari, Farhad, Rezaiemanesh, Alireza
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container_end_page 165
container_issue 2
container_start_page 160
container_title Laboratory medicine
container_volume 54
creator Hassani, Seyedeh Sara
Karamali, Negin
Rajabinejad, Misagh
Ashjari, Donya
Afshar Hezarkhani, Leila
Gorgin Karaji, Ali
Salari, Farhad
Rezaiemanesh, Alireza
description Abstract Objective Diabetic neuropathy (DN) is a type of nerve damage and the most common complication of diabetes. Regarding the association between endoplasmic reticulum (ER) stress with the pathogenesis of neuropathy, this study aims to examine binding immunoglobulin protein (BiP) gene expression and long noncoding RNA nuclear enriched abundant transcript 1 (NEAT1), miR-199a-5 as its regulator in the peripheral blood of DN patients compared to diabetic patients without neuropathy. Methods Peripheral blood samples were obtained from DN (n = 20) patients and diabetic patients without neuropathy (non-DN) (n = 20). After RNA extraction from peripheral blood mononuclear cells, reverse transcription-quantitative polymerase chain reaction was performed to evaluate RNA expression. Results The results showed that the expression level of NEAT1 and BiP genes in the DN group increased significantly compared to the non-DN group. Also, the expression level of miR-199a-5p in the DN group was significantly downregulated. Conclusion As a result, the axis of NEAT1, miR-199a-5p, and BiP may have a role in the DN pathogenesis.
doi_str_mv 10.1093/labmed/lmac082
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Regarding the association between endoplasmic reticulum (ER) stress with the pathogenesis of neuropathy, this study aims to examine binding immunoglobulin protein (BiP) gene expression and long noncoding RNA nuclear enriched abundant transcript 1 (NEAT1), miR-199a-5 as its regulator in the peripheral blood of DN patients compared to diabetic patients without neuropathy. Methods Peripheral blood samples were obtained from DN (n = 20) patients and diabetic patients without neuropathy (non-DN) (n = 20). After RNA extraction from peripheral blood mononuclear cells, reverse transcription-quantitative polymerase chain reaction was performed to evaluate RNA expression. Results The results showed that the expression level of NEAT1 and BiP genes in the DN group increased significantly compared to the non-DN group. Also, the expression level of miR-199a-5p in the DN group was significantly downregulated. Conclusion As a result, the axis of NEAT1, miR-199a-5p, and BiP may have a role in the DN pathogenesis.</description><identifier>ISSN: 0007-5027</identifier><identifier>EISSN: 1943-7730</identifier><identifier>DOI: 10.1093/labmed/lmac082</identifier><identifier>PMID: 36166353</identifier><language>eng</language><publisher>US: Oxford University Press</publisher><subject>Antisense RNA ; Care and treatment ; Diabetes Mellitus ; Diabetic neuropathies ; Diabetic Neuropathies - genetics ; Diabetics ; Genes ; Genetic transcription ; Humans ; Leukocytes, Mononuclear - metabolism ; MicroRNAs - genetics ; MicroRNAs - metabolism ; Protein binding ; RNA, Long Noncoding - genetics ; RNA, Long Noncoding - metabolism ; Type 2 diabetes</subject><ispartof>Laboratory medicine, 2023-03, Vol.54 (2), p.160-165</ispartof><rights>The Author(s) 2022. Published by Oxford University Press on behalf of American Society for Clinical Pathology. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com 2022</rights><rights>The Author(s) 2022. Published by Oxford University Press on behalf of American Society for Clinical Pathology. All rights reserved. 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Regarding the association between endoplasmic reticulum (ER) stress with the pathogenesis of neuropathy, this study aims to examine binding immunoglobulin protein (BiP) gene expression and long noncoding RNA nuclear enriched abundant transcript 1 (NEAT1), miR-199a-5 as its regulator in the peripheral blood of DN patients compared to diabetic patients without neuropathy. Methods Peripheral blood samples were obtained from DN (n = 20) patients and diabetic patients without neuropathy (non-DN) (n = 20). After RNA extraction from peripheral blood mononuclear cells, reverse transcription-quantitative polymerase chain reaction was performed to evaluate RNA expression. Results The results showed that the expression level of NEAT1 and BiP genes in the DN group increased significantly compared to the non-DN group. Also, the expression level of miR-199a-5p in the DN group was significantly downregulated. Conclusion As a result, the axis of NEAT1, miR-199a-5p, and BiP may have a role in the DN pathogenesis.</description><subject>Antisense RNA</subject><subject>Care and treatment</subject><subject>Diabetes Mellitus</subject><subject>Diabetic neuropathies</subject><subject>Diabetic Neuropathies - genetics</subject><subject>Diabetics</subject><subject>Genes</subject><subject>Genetic transcription</subject><subject>Humans</subject><subject>Leukocytes, Mononuclear - metabolism</subject><subject>MicroRNAs - genetics</subject><subject>MicroRNAs - metabolism</subject><subject>Protein binding</subject><subject>RNA, Long Noncoding - genetics</subject><subject>RNA, Long Noncoding - metabolism</subject><subject>Type 2 diabetes</subject><issn>0007-5027</issn><issn>1943-7730</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkbtv2zAQxokiQe0mXTsWBLIkg2xSfEmj8mgbwHAMI50JijrZLCTRFSW0_u9D1_YWoLjhHvh9h8N9CH2hZEZJzuaNKVuo5k1rLMnSD2hKc84SpRi5QFNCiEoESdUEfQrhV2x5LtOPaMIklZIJNkXl4z70sBkbMzjfYV_jhe82eOk76ysXq_WywMun4pXOW7dOaJ6bRMzv3QoXf13ArsOrqIRuCPiPG7b40ZkSBmfxEsbe78yw3V-jy9o0AT6f8hX6-e3p9eFHsnj5_vxQLBLLOR0SVsqaWMUzWhHKMgOlYYJyqShLBbe2tlllmbCcpimApYaBVWWVGQGpkJCxK3R73Lvr_e8RwqBbFyw0jenAj0GnimaScUIO6M0R3ZgGtOtqP_TGHnBdKCVFJpgUkZq9Q8WooHXWd1C7OH9PYHsf4ltrvetda_q9pkQf7NJHu_TJrij4ejp5_Dc_42d_InB3BPy4-9-yN0_Zna4</recordid><startdate>20230307</startdate><enddate>20230307</enddate><creator>Hassani, Seyedeh Sara</creator><creator>Karamali, Negin</creator><creator>Rajabinejad, Misagh</creator><creator>Ashjari, Donya</creator><creator>Afshar Hezarkhani, Leila</creator><creator>Gorgin Karaji, Ali</creator><creator>Salari, Farhad</creator><creator>Rezaiemanesh, Alireza</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-2551-705X</orcidid></search><sort><creationdate>20230307</creationdate><title>Dysregulation of Long Noncoding RNA NEAT1/miR-199a-5/BiP Axis in Patients with Diabetic Neuropathy</title><author>Hassani, Seyedeh Sara ; Karamali, Negin ; Rajabinejad, Misagh ; Ashjari, Donya ; Afshar Hezarkhani, Leila ; Gorgin Karaji, Ali ; Salari, Farhad ; Rezaiemanesh, Alireza</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c441t-3b6f0c7481d0138aeba35146713254ccfc8dc35c4122eec1a3ec7bd8a5e256e83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Antisense RNA</topic><topic>Care and treatment</topic><topic>Diabetes Mellitus</topic><topic>Diabetic neuropathies</topic><topic>Diabetic Neuropathies - genetics</topic><topic>Diabetics</topic><topic>Genes</topic><topic>Genetic transcription</topic><topic>Humans</topic><topic>Leukocytes, Mononuclear - metabolism</topic><topic>MicroRNAs - genetics</topic><topic>MicroRNAs - metabolism</topic><topic>Protein binding</topic><topic>RNA, Long Noncoding - genetics</topic><topic>RNA, Long Noncoding - metabolism</topic><topic>Type 2 diabetes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hassani, Seyedeh Sara</creatorcontrib><creatorcontrib>Karamali, Negin</creatorcontrib><creatorcontrib>Rajabinejad, Misagh</creatorcontrib><creatorcontrib>Ashjari, Donya</creatorcontrib><creatorcontrib>Afshar Hezarkhani, Leila</creatorcontrib><creatorcontrib>Gorgin Karaji, Ali</creatorcontrib><creatorcontrib>Salari, Farhad</creatorcontrib><creatorcontrib>Rezaiemanesh, Alireza</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Laboratory medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hassani, Seyedeh Sara</au><au>Karamali, Negin</au><au>Rajabinejad, Misagh</au><au>Ashjari, Donya</au><au>Afshar Hezarkhani, Leila</au><au>Gorgin Karaji, Ali</au><au>Salari, Farhad</au><au>Rezaiemanesh, Alireza</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dysregulation of Long Noncoding RNA NEAT1/miR-199a-5/BiP Axis in Patients with Diabetic Neuropathy</atitle><jtitle>Laboratory medicine</jtitle><addtitle>Lab Med</addtitle><date>2023-03-07</date><risdate>2023</risdate><volume>54</volume><issue>2</issue><spage>160</spage><epage>165</epage><pages>160-165</pages><issn>0007-5027</issn><eissn>1943-7730</eissn><abstract>Abstract Objective Diabetic neuropathy (DN) is a type of nerve damage and the most common complication of diabetes. Regarding the association between endoplasmic reticulum (ER) stress with the pathogenesis of neuropathy, this study aims to examine binding immunoglobulin protein (BiP) gene expression and long noncoding RNA nuclear enriched abundant transcript 1 (NEAT1), miR-199a-5 as its regulator in the peripheral blood of DN patients compared to diabetic patients without neuropathy. Methods Peripheral blood samples were obtained from DN (n = 20) patients and diabetic patients without neuropathy (non-DN) (n = 20). After RNA extraction from peripheral blood mononuclear cells, reverse transcription-quantitative polymerase chain reaction was performed to evaluate RNA expression. Results The results showed that the expression level of NEAT1 and BiP genes in the DN group increased significantly compared to the non-DN group. Also, the expression level of miR-199a-5p in the DN group was significantly downregulated. 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source Oxford University Press Journals All Titles (1996-Current); MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection
subjects Antisense RNA
Care and treatment
Diabetes Mellitus
Diabetic neuropathies
Diabetic Neuropathies - genetics
Diabetics
Genes
Genetic transcription
Humans
Leukocytes, Mononuclear - metabolism
MicroRNAs - genetics
MicroRNAs - metabolism
Protein binding
RNA, Long Noncoding - genetics
RNA, Long Noncoding - metabolism
Type 2 diabetes
title Dysregulation of Long Noncoding RNA NEAT1/miR-199a-5/BiP Axis in Patients with Diabetic Neuropathy
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