Serum albumin/hyaluronic acid nanoconjugate: Evaluation of concentration-dependent structural changes to form an efficient drug carrier particle

In actual work we clearly highlight in the first time the experimental factors (dominantly the concentration of the macromolecule components) influencing the formation of human serum albumin/hyaluronic acid (HSA/HyA) complex carrier particles which is not available in the literature despite the several published articles. We have shown that the charge compensation process of the oppositely charged macromolecules results in the formation of drug carrier particles (d ~ 200–350 nm), where the size, size distribution and structure can be largely controlled by changing the concentration of the macromolecule components and the experimental conditions (pH, reaction time, presence of inert salt etc.). Moreover, we firstly demonstrate the encapsulation capability of this optimized HSA/HyA carrier particles - which possess dominantly hydrophilic character - using vitamin D3 (D3) as a model compound having hydrophobic property, where the kinetic properties of the dissolution were studied as well. •Electrostatic and hydrophobic forces exist in serum albumin/hyaluronic acid system•The charge compensation process of macromolecules is affected by HyA concentration•Preparation of HSA/HyA particles can be strongly controlled by initial conditions•HSA/HyA nanocarrier facilitates better solubility and controlled dissolution of D3