Biocontrol treatment: Application of Bdellovibrio bacteriovorus HD100 against burn wound infection caused by Pseudomonas aeroginosa in mice

Owing to the high level of resistance to various antibiotics in bacteria causing burn wound infections, the alternative therapeutics is highly demanded. Bdellovibrio and like organisms (BALOs) seem to be a superb choice. In the present study, Bdellovibrio bacteriovorus HD100 was selected for treatin...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Burns 2023-08, Vol.49 (5), p.1181-1195
Hauptverfasser: Tajabadi, Fereshteh Heidari, Karimian, Sayed Morteza, Mohsenipour, Zeinab, Mohammadi, Sahar, Salehi, Mohammadreza, Sattarzadeh, Mahboubeh, Fakhari, Sima, Momeni, Mahnoush, Dahmardehei, Mohammad, Feizabadi, Mohammad Mehdi
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Owing to the high level of resistance to various antibiotics in bacteria causing burn wound infections, the alternative therapeutics is highly demanded. Bdellovibrio and like organisms (BALOs) seem to be a superb choice. In the present study, Bdellovibrio bacteriovorus HD100 was selected for treating burn wound infection caused by Pseudomonas aeruginosa strain PAO1 in a mouse model. In this experiment, two treatments, meropenem as antibiotic and B. bacteriovorus, were employed. Histopathology indicated an accelerated healing rate in both treatments in comparison with the control. Moreover, quantitative reverse transcription PCR (qRT-PCR) was applied to investigate the expression of tnf-α (tumor necrosis factor alpha), pdgf (platelet-derived growth factor), tgf-β1 (transforming growth factor beta1), ifn-γ (interferon gamma), vegf (vascular endothelial group factor), and col1 (collagen type 1). The results demonstrated that treating burn wound areas with Bdellovibrio not only decrease the inflammatory phase period, but also may improve the characteristics of proliferative phases of wound healing. In addition, a significant difference was explored between the two treatment groups in the regulation of all genes, except for pdgf revealed a significant up regulation in both treatment groups. The results disclose that Bdellovibrio attenuates P. aeruginosa in burn wounds infections and improves the wound healing process. •Application of Bdellovibrio bacteriovorous HD100 as a live antibiotic in mice burn wound infection.•Healing rate in Bdellovibrio treatments were accelerated.•The expression level of six genes: tnf-α, pdfg, tgf-β1, ifn-γ, vegf and col1 investigated.•The expression of vegf,tgf-β1 and col1genes in the Bdellovibrio treatments was significantly higher.•Bdellovibrio treatments reduce the period of inflammatory phase and improve the proliferative phases of wound healing.•Bdellovibrio attenuates P. aeruginosa in burn wounds infections.
ISSN:0305-4179
1879-1409
DOI:10.1016/j.burns.2022.08.020