A brief review on critical analytical aspects for quantification of ambroxol in biological samples

Ambroxol (AMB) is a member of the expectorant class, widely used as a secreolytic agent in patients to break up secretions. AMB is rapidly and effectively distributed from blood to tissue. The lungs have the highest concentration of AMB; accumulation of AMB in human lung tissue was detected at concentrations 15- to 20-fold greater than those reported in the circulation. Because of its wide range of actions and therapeutic applications may be worth looking into, particularly for respiratory symptoms, antioxidant, anti-inflammatory, influenza, and rhinovirus infections. Though several analytical methodologies have been established and confirmed for the AMB analysis in matrices of pharmaceutical and biological origins, novel sustainable, and economical methods are still to be choice of protocol to increase its sensitivity, reliability, and repeatability. Therefore, the present review offers an overview of critical analytical aspects regarding the HPLC, LC-MS/MS, HPTLC, capillary electrophoresis, spectrophotometry, and electrochemical methods for quantifying AMB in pharmaceutical and biological samples. Furthermore, this review will thoroughly discuss the physicochemical properties, stability, extraction conditions, instrumentation, and operational parameters of the targeted analyte. As a result, for the first time, this review complies with vital background information and an up-to-date interpretation of research undertaken by anticipated methodologies examined and implemented for the pharmaceutical analysis AMB. Bioanalytical and Analytical Methodologies Overview. [Display omitted] •Many analytical techniques for quantifying ambroxol in combination with other medicinal drugs have been investigated.•The most common techniques for pharmaceutical analysis of ambroxol, HPLC and LC-MS/MS, are briefly reviewed.•There are certain difficulties in developing new pharmacokinetics and drug-drug interactions methods.•For method efficiency and acceptance, physicochemical properties, sample preparation, internal standard, and the selection of important analytical variables are critical.