All‐in‐one microfluidic design to integrate vascularized tumor spheroid into high‐throughput platform

The development of a scalable and highly reproducible in vitro tumor microenvironment (TME) platform still sheds light on new insights into cancer metastasis mechanisms and anticancer therapeutic strategies. Here, we present an all‐in‐one injection molded plastic array three‐dimensional culture plat...

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Veröffentlicht in:Biotechnology and bioengineering 2022-12, Vol.119 (12), p.3678-3693
Hauptverfasser: Kim, Youngtaek, Ko, Jihoon, Shin, Nari, Park, Seonghyuk, Lee, Seung‐Ryeol, Kim, Suryong, Song, Jiyoung, Lee, Seokjun, Kang, Kyung‐Sun, Lee, Jeeyun, Jeon, Noo Li
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Sprache:eng
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Zusammenfassung:The development of a scalable and highly reproducible in vitro tumor microenvironment (TME) platform still sheds light on new insights into cancer metastasis mechanisms and anticancer therapeutic strategies. Here, we present an all‐in‐one injection molded plastic array three‐dimensional culture platform (All‐in‐One‐IMPACT) that integrates vascularized tumor spheroids for highly reproducible, high‐throughput experimentation. This device allows the formation of self‐assembled cell spheroids on a chip by applying the hanging drop method to the cell culture channel. Then, when the hydrogel containing endothelial cells and fibroblasts is injected, the spheroid inside the droplet can be patterned together in three dimensions along the culture channel. In just two steps above, we can build a vascularized TME within a defined area. This process does not require specialized user skill and minimizes error‐inducing steps, enabling both reproducibility and high throughput of the experiment. We have successfully demonstrated the process, from spheroid formation to tumor vascularization, using patient‐derived cancer cells (PDCs) as well as various cancer cell lines. Furthermore, we performed combination therapies with Taxol (paclitaxel) and Avastin (bevacizumab), which are used in standard care for metastatic cancer. The All‐in‐One IMPACT is a powerful tool for establishing various anticancer treatment strategies through the development of a complex TME for use in high‐throughput experiments. Kim and coworkers developed a microfluidic platform capable of one‐step modeling from tumor spheroids to their vascularization. The authors validated platform using patient samples as well as cancer cell‐line. Also, Efficacy evaluation of anticancer combination therapies was performed in the platform by establishing a tumor microenvironment. The platform provides translational research, bridging engineering and clinical practice.
ISSN:0006-3592
1097-0290
DOI:10.1002/bit.28221