Methods to determine biotin-binding capacity of streptavidin-coated magnetic particles

Two assays to determine the biotin-binding capacity of streptavidin magnetic particles are described and compared. The two assays are based on the use of biotinylated alkaline phosphatase and biotinylated fluorescein, respectively. Also, an assay for bound protein is presented. When the biotin-bindi...

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Veröffentlicht in:	Journal of magnetism and magnetic materials 1999, Vol.194 (1), p.69-75
Hauptverfasser:	Dorgan, Lonnie, Magnotti, Ralph, Hou, Janming, Engle, Terri, Ruley, Kevin, Shull, Bruce
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotin binding Fundamental and applied biological sciences. Psychology Fundamental immunology Magnetic microparticles Molecular immunology Streptavidin Techniques
Online-Zugang:	Volltext
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Beschreibung
Zusammenfassung:	Two assays to determine the biotin-binding capacity of streptavidin magnetic particles are described and compared. The two assays are based on the use of biotinylated alkaline phosphatase and biotinylated fluorescein, respectively. Also, an assay for bound protein is presented. When the biotin-binding methods are combined with the protein assay, the specific activity can be determined. The fluorescent version is used to compare the streptavidin magnetic particles from several manufacturers.
ISSN:	0304-8853
DOI:	10.1016/S0304-8853(98)00563-0