Cas12a‐based on‐site, rapid detection of genetically modified crops
A CRISPR/LbCas12a‐based nucleic acid detection method that uses crude leaf extracts as samples and is rapid (≤40 min for a full run) and highly sensitive (0.01%) can be used to monitor genetically modified organisms in the field.
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| Veröffentlicht in: | Journal of integrative plant biology 2022-10, Vol.64 (10), p.1856-1859 |
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| container_end_page | 1859 |
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| container_issue | 10 |
| container_start_page | 1856 |
| container_title | Journal of integrative plant biology |
| container_volume | 64 |
| creator | Duan, Zhiqiang Yang, Xiaoliang Ji, Xingkun Chen, Ying Niu, Xiaomu Guo, Anping Zhu, Jian‐Kang Li, Feng Lang, Zhaobo Zhao, Hui |
| description | A CRISPR/LbCas12a‐based nucleic acid detection method that uses crude leaf extracts as samples and is rapid (≤40 min for a full run) and highly sensitive (0.01%) can be used to monitor genetically modified organisms in the field. |
| doi_str_mv | 10.1111/jipb.13342 |
| format | Article |
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| identifier | ISSN: 1672-9072 |
| ispartof | Journal of integrative plant biology, 2022-10, Vol.64 (10), p.1856-1859 |
| issn | 1672-9072 1744-7909 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2702177916 |
| source | Wiley Journals; Alma/SFX Local Collection |
| subjects | CRISPR Genetic modification Genetically engineered microorganisms Genetically engineered organisms Genetically modified organisms Nucleic acids Plant extracts |
| title | Cas12a‐based on‐site, rapid detection of genetically modified crops |
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