Cas12a‐based on‐site, rapid detection of genetically modified crops

Cas12a‐based on‐site, rapid detection of genetically modified crops

A CRISPR/LbCas12a‐based nucleic acid detection method that uses crude leaf extracts as samples and is rapid (≤40 min for a full run) and highly sensitive (0.01%) can be used to monitor genetically modified organisms in the field.

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Bibliographische Detailangaben
Veröffentlicht in:Journal of integrative plant biology 2022-10, Vol.64 (10), p.1856-1859
Hauptverfasser: Duan, Zhiqiang, Yang, Xiaoliang, Ji, Xingkun, Chen, Ying, Niu, Xiaomu, Guo, Anping, Zhu, Jian‐Kang, Li, Feng, Lang, Zhaobo, Zhao, Hui
Format: Artikel
Sprache:eng
Schlagworte:
CRISPR
Genetic modification
Genetically engineered microorganisms
Genetically engineered organisms
Genetically modified organisms
Nucleic acids
Plant extracts
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Beschreibung
Zusammenfassung:A CRISPR/LbCas12a‐based nucleic acid detection method that uses crude leaf extracts as samples and is rapid (≤40 min for a full run) and highly sensitive (0.01%) can be used to monitor genetically modified organisms in the field.
ISSN:1672-9072
1744-7909
DOI:10.1111/jipb.13342