Root Hair Apex is the Key Site for Symplastic Delivery of Graphene into Plants

Uptake kinetics and delivery mechanisms of nanoparticles (NPs) in crop plants need to be urgently understood for the application of nanotechnology in agriculture as delivery systems for eco-friendly nanoagrochemicals. Here, we investigated the uptake kinetics, translocation pathway, and key internalization process of graphene in wheat (Triticum aestivum L.) by applying three specific hydroponic cultivation methods (submerging, hanging, and split-root). Quantification results on the uptake of carbon-14 radiolabeled graphene in each tissue indicated that graphene could enter the root of wheat and further translocate to the shoot with a low delivery rate (