Combined effects of biochar and biogas slurry on soil nitrogen transformation rates and N2O emission in a subtropical poplar plantation

It has been widely accepted that biochar has a great potential of mitigating soil nitrous oxide (N2O) emission. However, the underlying mechanism about how biochar affects nitrogen transformation and the pathways of soil N2O production is under discussion. A 15N-tracer incubation experiment was conducted to investigate the short-term effects of biochar on soil N transformation rates and source partitioning of N2O emissions in soils from a poplar plantation system. A two-factor experimental design was adopted using biogas digestate slurry and biochar as soil amendments. In total, there were 12 treatments, including three rates of biochar: B0 (control), B2 (80 t ha−1), and B3 (120 t ha−1), and four rates of biogas digestate slurry: C (0 m3 ha−1), L (125 m3 ha−1), M (250 m3 ha−1), and H (375 m3 ha−1). We observed significantly lower rates of net nitrification (Nn) and mineralization (Mn) in biochar-treated soils. The 15N tracer analysis revealed a significant decrease in gross autotrophic (ONH4), heterotrophic nitrification (ONrec), and mineralization (MNorg) rates while an increase in gross immobilization (INH4 and INO3) rates in biochar amended soils. When biogas slurry was applied, biochar only significantly reduced ONH4 except in the moderate slurry treatment. Regardless of the slurry application, biochar consistently suppressed N2O emission by 58–89 %, and nitrification was the dominant pathway accounting contributing >90 % to cumulative N2O emissions. Moreover, soil cumulative N2O emissions significantly negatively correlated with soil ammonium contents and positively with MNorg, Mn, and Nn, showing that biochar decreased N2O emission via a reducing effect on nitrification rates and associated N2O emissions. Our results also highlight that application of N fertilizer greatly influence the biochar's impacts on soil N transformation rates and N2O emission, calling for further studies on their interactions to develop mitigate options and to improve N use efficiency. [Display omitted] •Biochar significantly decreased net nitrification and mineralization rates.•Biochar alone decreased ONH4, ONrec and MNorg rates while increased INH4 and INO3 rates.•With slurry applied, biochar only significantly reduced autotrophic nitrification rates.•Biochar suppressed N2O emission by 58–89 %, and nitrification was the major pathway.