The effects of microgravity on induced mutation in Escherichia coli and Saccharomyces cerevisiae

We examined whether microgravity influences the induced-mutation frequencies through in vivo experiments during space flight aboard the space shuttle Discovery (STS-91). We prepared dried samples of repair-deficient strains and parental strains of Escherichia ( E.) coli and Saccharomyces ( S.) cerev...

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Veröffentlicht in:Advances in space research 2001-01, Vol.28 (4), p.555-561
Hauptverfasser: Takahashi, A., Ohnishi, K., Takahashi, S., Masukawa, M., Sekikawa, K., Amano, T., Nakano, T., Nagaoka, S., Ohnishi, T.
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Sprache:eng
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Zusammenfassung:We examined whether microgravity influences the induced-mutation frequencies through in vivo experiments during space flight aboard the space shuttle Discovery (STS-91). We prepared dried samples of repair-deficient strains and parental strains of Escherichia ( E.) coli and Saccharomyces ( S.) cerevisiae given DNA damage treatment. After culture in space, we measured the induced-mutation frequencies and SOS-responses under microgravity. The experimental findings indicate that almost the same induced-mutation frequencies and SOS-responses of space samples were observed in both strains compared with the ground control samples. It is suggested that microgravity might not influence induced-mutation frequencies and SOS-responses at the stages of DNA replication and/or DNA repair. In addition, we developed a new experimental apparatus for space experiments to culture and freeze stocks of E. coli and S. cerevisiae cells.
ISSN:0273-1177
1879-1948
DOI:10.1016/S0273-1177(01)00391-X