Amyloid‐Mediated Remineralization in Pit and Fissure for Caries Preventive Therapy

The pits and fissures of teeth have high caries susceptibility, and sealing these areas is considered as an effective method to prevent caries. However, long‐term caries prophylaxis cannot be maintained because of the negative effects derived from the technical sensitivity and disadvantages of sealing materials. Herein, a new strategy is proposed to occlude fossae by amyloid‐mediated biomimetic remineralization. In contrast to conventional inward blocking from the outside of fossae, amyloid‐mediated biomimetic mineralization delivers an amyloid‐like protein nanofilm into the deepest zone of the fossae and induces the formation of remineralized enamel inside. Such assembly from lysozyme conjugated with poly (ethylene glycol) enriches the interface with strongly bonded ionsand directs in situ nucleation to achieve enamel epitaxial growth. Not only is the structure of the enamel‐like crystalline hydroxyapatite layer but also its mechanical stability is similar to that of natural enamel. Furthermore, the layer shows good biocompatibility and antibacterial properties. On the basis of the findings, it is demonstrated that amyloid‐like protein aggregation may provide an enamel remineralization strategy to modify the current clinically available methods of pit and fissure sealing and shows great promise in preventing caries. The amyloid aggregation of lyso‐poly (ethylene glycol) emulsion provides a smart medium to induce in situ remineralization‐based sealing in the deepest zone and reconstructs the structure of pit and fissure. This study is the first attempt to achieve pit and fissure sealing by using an enamel regeneration strategy, which provides a solid foundation for caries prevention.