Beyond the centrosome: The mystery of microtubule organising centres across mammalian preimplantation embryos

Mammalian preimplantation embryogenesis depends on the spatio-temporal dynamics of the microtubule cytoskeleton to enable exceptionally fast changes in cell number, function, architecture, and fate. Microtubule organising centres (MTOCs), which coordinate the remodelling of microtubules, are therefore of fundamental significance during the first days of a new life. Despite its indispensable role during early mammalian embryogenesis, the origin of microtubule growth remains poorly understood. In this review, we summarise the most recent discoveries on microtubule organisation and function during early human embryogenesis and compare these to innovative studies conducted in alternative mammalian models. We emphasise the differences and analogies of centriole inheritance and their role during the first cleavage. Furthermore, we highlight the significance of non-centrosomal MTOCs for embryo viability and discuss the potential of novel in vitro models and light-inducible approaches towards unravelling microtubule formation in research and assisted reproductive technologies. •Centrosome dynamics during the first mitotic division influence the vulnerability of human embryos to aneuploidy.•Non-human mammalian embryogenesis models display plasticity in their use of non-centrosomal microtubule organising centres.•Comparative analysis of the available in vivo and in vitro models of mammalian embryogenesis.