Sulphonated graphene catalyst incorporation with sludge enhanced the microbial activities for biomethanization of crude rice straw

[Display omitted] •Sulphonated graphene catalyst incorporation with sludge delignified rice straw.•Solubilization of rice straw is increased with addition of anaerobes and catalyst.•Sulphonated graphene catalyst boosted the microbial activities degrading rice straw.•Crenarchaeota, Euryarchaeota and Parvarchaeota phyla increased with catalyst addition.•Biomethanization of rice straw by anaerobes/catalyst could be replicated in other biomass. Biomethanization of crude rice straw (RS) was enhanced by a coupled effectiveness of sulphonated graphene (SGR) with sludge rich anaerobes (SRA). A reduction of 19.2 ± 1.32% for cellulose, 40.8 ± 3.7% for hemicellulose and 30.8 ± 2.4% for lignin was achieved with addition of SRA after fermentation of 60 days. The abundance of hydrolytic microbes in SRA i.e. Acidobacteria, Bacteroidetes, Chloroflexi and Proteobacteria caused RS structure liquefaction and dissolution. The reduction of cellulose, hemicellulose and lignin boosted to 92.3 ± 1.5, 84.9 ± 3.5 and 97.0 ± 1.8% respectively with SGR catalyst addition of 100 mg/gVS. Reducing sugars, phenols and volatile fatty acids (VFAs) were subsequently utilized by bacteria and archaea species of Methanosphaera, Methanocella, Candidatus Methanoregula, Methanolinea and Methanosaeta. The biogas yield was 92 ± 3.1 mL/gRS and methane content amounted to 68 ± 4.6% % at SGR catalyst of 80 mg/gVS. These findings show the potential of using SRA/SGR to improve the RS fermentation with a novel application for biogas productivity.