Determination of the binding reaction between avidin and biotin by relaxation measurements of magnetic nanoparticles

Determination of the binding reaction between avidin and biotin by relaxation measurements of magnetic nanoparticles

The binding between avidin and biotinylated magnetic iron oxide nanoparticles can be monitored by means of magnetic relaxation measurements. The resultant signal, caused by particle aggregates, has Brownian and Néel components. The time constant and amplitude of Brownian relaxation were determined b...

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Veröffentlicht in:Journal of magnetism and magnetic materials 1999-04, Vol.194 (1), p.62-68
Hauptverfasser: Kötitz, R, Weitschies, W, Trahms, L, Brewer, W, Semmler, W
Format: Artikel
Sprache:eng
Schlagworte:
Biological and medical sciences
Biological binding reaction
Brownian relaxation
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Molecular immunology
Néel relaxation
Techniques
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Zusammenfassung:The binding between avidin and biotinylated magnetic iron oxide nanoparticles can be monitored by means of magnetic relaxation measurements. The resultant signal, caused by particle aggregates, has Brownian and Néel components. The time constant and amplitude of Brownian relaxation were determined by a fit algorithm which also yielded Néel amplitudes proportional to the avidin content of the samples.
ISSN:0304-8853
DOI:10.1016/S0304-8853(98)00580-0