Preparation and characterization of affinity magnetoliposomes useful for the detection of antiphospholipid antibodies

The preparation of phosphatidylcholine-based magnetoliposomes containing the affinity target phospholipids, phosphatidylethanolamine (PE), phosphatidylserine (PS), and cardiolipin (CL), was studied. These magnetoliposomes are potentially useful as adsorbents for the detection of antiphospholipid antibodies. The dry-lipid film hydration method was used to prepare magnetoliposomes containing CL, while PE- or PS-containing magnetoliposomes were prepared by adsorption of phospholipids on magnetite. Both types of magnetoliposomes were characterized by iron and phospholipid content, mean diameter, size distribution, and retention efficiency in a high-gradient magnetic field.