A three-eyed radiodont with fossilized neuroanatomy informs the origin of the arthropod head and segmentation

In addition to being among the most iconic and bizarre-looking Cambrian animals, radiodonts are a group that offers key insight into the acquisition of the arthropod body plan by virtue of their phylogenetic divergence prior to all living members of the phylum. Nonetheless, radiodont fossils are rare and often fragmentary, and contentions over their interpretation have hindered resolution of important evolutionary conundrums. Here, we describe 268 specimens of Stanleycaris hirpex from the Cambrian Burgess Shale, including many exceptionally preserved whole-body specimens, informing the most complete reconstruction of a radiodont to date. The trunk region of Stanleycaris has up to 17 segments plus two pairs of filiform caudal blades. The recognition of dorsal sclerotic segmentation of the trunk cuticle and putative unganglionated nerve cords provides new insight into the relative timing of acquisition of segmental traits, the epitome of the arthropod body plan. In addition to the pair of stalked lateral eyes, the short head unexpectedly bears a large median eye situated behind a preocular sclerite on an anteriorly projecting head lobe. Upon re-evaluation, similar median eyes can be identified in other Cambrian panarthropods demonstrating a deep evolutionary continuity. The exquisitely preserved brain of Stanleycaris is consistent with the hypothesized deutocerebral innervation of the frontal appendages, reconciling neuroanatomical evidence with external morphology in support of an ancestrally bipartite head and brain for arthropods. We propose that the integration of this bipartite head prior to the acquisition of most segmental characters exclusively in the arthropod trunk may help explain its developmental differentiation. [Display omitted] •The radiodont Stanleycaris is reconstructed from a collection of exquisite fossils•Stanleycaris has three eyes, demonstrating evolutionary continuity of median eyes•Radiodont frontal appendages are innervated by the second brain neuromere•Fossils support early differentiation between arthropod head and trunk segmentation Many early arthropods are known only from rare, fragmentary fossils. Moysiuk and Caron describe hundreds of new, exceptional Cambrian fossils of the radiodont Stanleycaris hirpex, revealing surprising features such as a median eye and preserved neuroanatomy. They set out a new theory for the evolution of key features of the arthropod body plan.